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Table 2 in Discovery of Cytospora species associated with canker disease of tree hosts from Mount Dongling of China

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Table 2. Genes used in this study with PCR primers, primer DNA sequence, optimal annealing temperature and corresponding references.

LocusDefinitionPrimersPrimer DNA sequence (5'-3')Optimal annealing temp (°C)References of primers used
ITSinternal transcribed spacer of ribosomal RNAITS1TCCGTAGGTGAACCTGCGG51White et al. 1990
ITS4TCCTCCGCTTTTGATATGC
LSUlarge subunit of ribosomal RNALRORACCCGCTGAACTTAAGC55Vilgalys and Hester 1990
LR7TACTACCACCAAGATCT
actactinACT-512FATGTGCAAGGCCGGTTTCGC61Carbone and Kohn 1999
ACT-783RTACGAGTCCTTCTGGCCCAT
rpb2RNA polymerase II second largest subunitRPB2-5FGA(T/C)GA(T/C)(A/C)G(A/T)GATCA(T/C)TT(T/C)GG52Liu et al. 1999
RPB2-7cRCCCAT(A/G)GCTTG(T/C)TT(A/G)CCCAT
tef-1αtranslation elongation factor 1-alphaEF1-668FCGGTCACTTGATCTACAAGTGC55Alves et al. 2008
EF1-1251RCCTCGAACTCACCAGTACCG
tub2beta-tubulinBt2aGGTAACCAAATCGGTGCTGCTTTG55Glass and Donaldson 1995
Bt2bACCCTCAGTGTAGTGACCCTTGGC

Notes

Published as part of Zhu, Haiyan, Pan, Meng, Bezerra, Jadson D. P., Tian, Chengming & Fan, Xinlei, 2020, Discovery of Cytospora species associated with canker disease of tree hosts from Mount Dongling of China, pp. 97 in MycoKeys 62 on page 97, DOI: 10.3897/mycokeys.62.47854

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http://table.plazi.org/id/63470480C9B175241377C23FA2860989

Cites
Publication: 10.1016/B978-0-12-372180-8.50042-1 (DOI)
Publication: 10.1128/JB.172.8.4238-4246.1990 (DOI)
Publication: 10.1080/00275514.1999.12061051 (DOI)
Publication: 10.1093/oxfordjournals.molbev.a026092 (DOI)
Publication: 10.1128/AEM.61.4.1323-1330.1995 (DOI)
Is part of
Journal article: 10.3897/mycokeys.62.47854 (DOI)
Journal article: urn:lsid:plazi.org:pub:0CDEF6D5DE4451A181F8818FFC66ACD9 (LSID)
Journal article: http://publication.plazi.org/id/0CDEF6D5DE4451A181F8818FFC66ACD9 (URL)
Journal article: http://zenodo.org/record/null (URL)
Journal article: 10.3897/mycokeys.62.47854 (DOI)

References

  • White, TJ, Bruns, T, Lee, S, Taylor, J, 1990. Amplifcation and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR Protocols: a guide to methods and applications 18: 315 - 322, DOI: https://doi.org/10.1016/B978-0-12-372180-8.50042-1
  • Vilgalys, R, Hester, M, 1990. Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. Journal of Bacteriology 172: 4238 - 4246, DOI: https://doi.org/10.1128/JB.172.8.4238-4246.1990
  • Carbone, I, Kohn, LM, 1999. A method for designing primer sets for speciation studies in filamentous ascomycetes. Mycologia 91: 553 - 556, DOI: https://doi.org/10.1080/00275514.1999.12061051
  • Liu, YJ, Whelen, S, Hall, BD, 1999. Phylogenetic relationships among ascomycetes: evidence from an RNA polymerse II subunit. Molecular Biology and Evolution 16: 1799 - 1808, DOI: https://doi.org/10.1093/oxfordjournals.molbev.a026092
  • Alves, A, Crous, PW, Correia, A, Phillips, AJL, 2008. Morphological and molecular data reveal cryptic speciation in Lasiodiplodia theobromae. Fungal Diversity 28: 1 - 13
  • Glass, NL, Donaldson, GC, 1995. Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes. Applied and Environmental Microbiology 61: 1323 - 1330, DOI: https://doi.org/10.1128/AEM.61.4.1323-1330.1995