Published December 22, 2025
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A revision of the Mallomonas guttata species complex (Synurales, Chrysophyceae) based on morphological and molecular criteria
Authors/Creators
- 1. Severtsov Institute of Ecology and Evolution, Russian Academy of Sciences, Moscow, Russia
- 2. Institute for Cellular and Intracellular Symbiosis, Orenburg Federal Research Center, Ural Branch, Russian Academy of Sciences, Orenburg, Russia
- 3. Joint Vietnam-Russia Tropical Science and Technology Research Center, Hanoi, Vietnam
- 4. Central MIndanao University, Maramag, Philippines
- 5. T.I. Vyazemsky Karadag Scientific Station, Natural Reserve of the Russian Academy of Sciences, Feodosiya, Russia
Description
Mallomonas guttata species complex was investigated in a tropical region using electron microscopy and molecular phylogeny. A study of six algal cultures belonging to the M. guttata morphotype revealed two main clades based on two datasets (SSU rDNA+rbcL cpDNA, and ITS rDNA). This work establishes the phylogenetic position of M. guttata for the first time. We propose the new species, M. monilifera sp. nov., for organisms from the second clade, which were distinguished by the presence of a rim around the pits located on the shield on their scales and showed considerable genetic distances from the M. guttata clade. Within this new species, the strains formed two subclades, for which we propose two subspecies. Scales of the new species were also found in several water bodies on Mindanao Island (Philippines).
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References
- Andersen RA (2005) Algal Culturing Techniques. Elsevier Academic Press, Oxford, 589 pp.
- Asmund B, Kristiansen J (1986) The genus Mallomonas (Chrysophyceae). A taxonomic survey based on the ultrastructure of silica scales and bristles. Opera Botanica 85: 1–128.
- Belevich TA, Ilyash LV, Milyutina IA, Logacheva MD, Goryunov DV, Troitsky AV (2015) Metagenomic analyses of White Sea picoalgae: First data. Biochemistry. Biokhimiia 80: 1514–1521. https://doi.org/10.1134/S0006297915110140
- Čertnerová D, Čertner M, Škaloud P (2019) Molecular phylogeny and evolution of phenotype in silica-scaled chrysophyte genus Mallomonas. Journal of Phycology 55(4): 912–923. https://doi.org/10.1111/jpy.12882
- Chernomor O, von Haeseler A, Minh BQ (2016) Terrace aware data structure for phylogenomic inference from supermatrices. Systematic Biology 65: 997–1008. https://doi.org/10.1093/sysbio/syw037
- Choi B, Son M, Kim JI, Shin W (2013) Taxonomy and phylogeny of the genus Cryptomonas (Cryptophyceae, Cryptophyta) from Korea. Algae - Korean Phycological Society 28(4): 307–330. https://doi.org/10.4490/algae.2013.28.4.307
- Cronberg G, Hickel B (1985) Mallomonas fenestrata sp. nov. and M. perforata sp. nov. (Chrysophyceae, Mallomonadaceae) from tropical lakes. Nordic Journal of Botany 5: 105–110. https://doi.org/10.1111/j.1756-1051.1985.tb02079.x
- Darriba D, Taboada GL, Doallo R, Posada D (2012) jModelTest 2: More models, new heuristics and parallel computing. Nature Methods 9(8): 1–772. https://doi.org/10.1038/nmeth.2109
- Daugbjerg N, Andersen RA (1997) Phylogenetic analysis of the rbcL sequences from haptophytes and heterokont algae suggest their chloroplasts are unrelated. Molecular Biology and Evolution 14(12): 1242–1251. https://doi.org/10.1093/oxfordjournals.molbev.a025733
- Doan-Nhu H, Tinh TT, Gusev ES, Kulikovskiy MS, Phan-Tan L, Nguyen-Ngoc L (2021) Taxonomic Composition of Silica-Scaled Chrysophytes in a Tropical Mountain Reservoir. Inland Water Biology 14: 490–499. https://doi.org/10.1134/S1995082921050060
- Gusev ES (2013) Studies on synurophycean algae from mangrove wetlands (Vietnam). Nova Hedwigia. Beiheft 142: 87–98.
- Gusev E, Martynenko N (2022) Diversity of Silica‐Scaled Chrysophytes in Central Vietnam. Water (Basel) 14: 1–21. https://doi.org/10.3390/w14010065
- Gusev ES, Nguyen THT (2011) Silica-scaled chrysophytes (Chrysophyceae and Synurophyceae) from Vietnam (Khanh Hoa and Quang Nam provinces). Nova Hedwigia. Beiheft 93: 191–199. https://doi.org/10.1127/0029-5035/2011/0093-0191
- Gusev ES, Doan-Nhu H, Nguyen-Ngoc L (2017) Silica-Scaled Chrysophytes from Cat Tien National Park (Dong Nai Province, Vietnam). Nova Hedwigia. Beiheft 105(3–4): 347–364. https://doi.org/10.1127/nova_hedwigia/2017/0416
- Gusev ES, Čertnerová D, Škaloudová M, Škaloud P (2018) Exploring Cryptic Diversity and Distribution Patterns in the Mallomonas kalinae/rasilis Species Complex with a Description of a New Taxon—Mallomonas furtiva sp. nov. The Journal of Eukaryotic Microbiology 65: 38–47. https://doi.org/10.1111/jeu.12427
- Gusev ES, Doan-Nhu H, Nguyen-Ngoc L, Guseva EE, Luom PT (2019) Silica-scaled chrysophytes from Cam Ranh Peninsula (Khanh Hoa Province, Vietnam). Nova Hedwigia. Beiheft 148: 63–76. https://doi.org/10.1127/nova-suppl/2019/077
- Gusev E, Martynenko N, Tran H (2021) Studies on Algae from the Order Synurales (Chrysophyceae) in Northern Vietnam. Diversity 13: 1–14. https://doi.org/10.3390/d13110602
- Gusev E, Guseva E, Dien TD, Kulikovskiy M (2022a) Flora of silica-scaled chrysophytes (Chrysophyceae) of two provinces in Southern Vietnam. Inland Water Biology 15: 205–216. https://doi.org/10.1134/S1995082922030063
- Gusev E, Martynenko N, Kapustin D, Doan-Nhu H, Nguyen-Ngoc L (2022b) Diversity of silica-scaled chrysophytes of two tropical islands: Phu Quoc and Con Son (Viet Nam). Life 12: 1611. https://doi.org/10.3390/life12101611
- Gusev E, Martynenko N, Shkurina N, Huan PT, Dien TD, Thanh NTH (2023) An Annotated Checklist of Algae from the Order Synurales (Chrysophyceae) of VietNam. Diversity 15: 183. https://doi.org/10.3390/d15020183
- Gusev E, Shkurina N, Martynenko N (2024a) Phylogenetic position, typification and emended description of Mallomonas matvienkoae (Chrysophyceae, Synurales). Phytotaxa 660(2): 112–122. https://doi.org/10.11646/phytotaxa.660.2.2
- Gusev ES, Martynenko NA, Podunay YA, Kuzmin DV (2024b) Morphology, phylogenetic position and distribution of Mallomonas mangofera and Mallomonas foveata comb. et stat. nov. (Synurales, Chrysophyceae). Phytotaxa 662: 224–238. https://doi.org/10.11646/phytotaxa.662.3.2
- Gusev E, Martynenko N, Tran H, Podunay Y (2024c) Two new species of the Mallomonas mangofera species complex (Synurales, Chrysophyceae) from the tropics based on morphological and molecular studies. Phytotaxa 678: 017–032. https://doi.org/10.11646/phytotaxa.678.1.2
- Gusev ES, Podunay YA, Martynenko NA, Tran H, Kuzmin DV (2024d) Mallomonas gemina comb. et stat. nov. (Synurales, Chrysophyceae)—A new combination based on molecular studies. Phytotaxa 665: 221–232. https://doi.org/10.11646/phytotaxa.665.3.4
- Gusev ES, Safronova TV, Podunay YA, Tran H, Martynenko NA (2024e) Hidden diversity in the Mallomonas pseudomatvienkoae species complex (Synurales, Chrysophyceae) and description of two new species. Phycologia 63(6): 544–557. https://doi.org/10.1080/00318884.2024.2430034
- Gusev ES, Shkurina NA, Tran H, Martynenko NA (2025a) Revision of the Mallomonas splendens species clade (Chrysophyceae, Synurales) based on morphological and molecular data. Phytotaxa 708(3): 241–259. https://doi.org/10.11646/phytotaxa.708.3.1
- Gusev ES, Ignatenko ME, Lituanas CR, Gumapac KR, Martynenko NA, Efeykin BD (2025b) (in press) Diversity of silica-scaled chrysophytes (Chrysophyceae) from Mindanao Island (Philippines) with the description of Mallomonas pseudopustula sp. nov. PhytoKeys.
- Hamby RK, Sims L, Issel L, Zimmer E (1988) Direct ribosomal RNA sequencing: Optimization of extraction and sequencing methods for work with higher plants. Plant Molecular Biology Reporter 6: 175–192. https://doi.org/10.1007/BF02669591
- Hansen P (1996) Silica-scaled Chrysophyceae and Synurophyceae from Madagascar. Archiv für Protistenkunde l 47: 145–172. https://doi.org/10.1016/S0003-9365(96)80030-3
- Hao J, Nan F, Lü J, Liu Q, Liu X, Xie S, Feng J (2024) Morphology and phylogenetic placement of Mallomonas vietnamica (Synurales, Chrysophyceae) from China. Journal of Oceanology and Limnology 42: 1325–1335. https://doi.org/10.1007/s00343-023-3124-3
- Jeong M, Kim JI, Jo BY, Kim HS, Siver PA, Shin W (2019) Surviving the marine environment: Two new species of Mallomonas (Synurophyceae). Phycologia 58(3): 276–286. https://doi.org/10.1080/00318884.2019.1565718
- Jo BY, Shin W, Boo SM, Kim HS, Siver PA (2011) Studies on ultrastructure and three-gene phylogeny of the genus Mallomonas (Synurophyceae). Journal of Phycology 47(2): 415–425. https://doi.org/10.1111/j.1529-8817.2010.00953.x
- Jo BY, Shin W, Kim HS, Siver PA, Andersen RA (2013) Phylogeny of the genus Mallomonas (Synurophyceae) and descriptions of five new species on the basis of morphological evidence. Phycologia 52(3): 266–278. https://doi.org/10.2216/12-107.1
- Katana A, Kwiatowski J, Spalik K, Zakryś B, Szalacha E, Szymańska H (2001) Phylogenetic position of Koliella (Chlorophyta) as inferred from nuclear and chloroplast small subunit rDNA. Journal of Phycology 37(3): 443–451. https://doi.org/10.1046/j.1529-8817.2001.037003443.x
- Katoh K, Rozewicki J, Yamada KD (2019) MAFFT online service: Multiple sequence alignment, interactive sequence choice and visualization. Briefings in Bioinformatics 20(4): 1160–1166. https://doi.org/10.1093/bib/bbx108
- Kim HS, Kim JH, Shin W, Jo BY (2014) Mallomonas elevata sp. nov. (Synurophyceae), a new scaled Chrysophyte from Jeju Island, South Korea. Nova Hedwigia. Beiheft 98: 89–102. https://doi.org/10.1127/0029-5035/2013/0138
- Knotek P, Pusztai M, Jadrná I, Škaloud P (2025) Expanding our knowledge of Synurales (Chrysophyceae) in Florida, United States: Proposing three novel Mallomonas taxa. Journal of Phycology 00: 1–19. https://doi.org/10.1111/jpy.70062
- Kristiansen J, Preisig HR (2007) Chrysophyte and Haptophyte Algae 2: Teil/2nd Part: Synurophyceae. Spektrum Akademischer Verlag, Heidelberg, 252 pp.
- Kristiansen J, Tong D (1989) Studies on silica-scaled chrysophytes from Wuhan, Hangzhou and Beijing, P.R. China. Nova Hedwigia. Beiheft 49(1–2): 183–202.
- Kristiansen J, Tong D, Olrik K (1990) Silica-scaled chrysophytes from Korea, a preliminary study. Nordic Journal of Botany 9: 685–691. https://doi.org/10.1111/j.1756-1051.1990.tb00561.x
- Kumar S, Stecher G, Li M, Knyaz C, Tamura K (2018) MEGA X: Molecular Evolutionary Genetics Analysis across computing platforms. Molecular Biology and Evolution 35: 1547–1549. https://doi.org/10.1093/molbev/msy096
- Martynenko N, Shkurina N, Gusev E (2024) Description of the new species Mallomonas okhapkinii (Synurales, Chrysophyceae) based on morphological and molecular data. Phytotaxa 670(1): 63–71. https://doi.org/10.11646/phytotaxa.670.1.6
- McFadden GI, Melkonian M (1986) Use of Hepes buffer for microalgal culture media and fixation for electron microscopy. Phycologia 25: 551–557. https://doi.org/10.2216/i0031-8884-25-4-551.1
- Minh BQ, Schmidt HA, Chernomor O, Schrempf D, Woodhams MD, von Haeseler A, Lanfear R (2020) IQ-TREE 2: New models and efficient methods for phylogenetic inference in the genomic era. Molecular Biology and Evolution 37: 1530–1534. https://doi.org/10.1093/molbev/msaa015
- Nemcova Y, Diaz-Pulido G (2023) Floristic and ecological insights into silica-scaled chrysophytes in southeastern Queensland, Australia. Plant Systematics and Evolution 309: 43. https://doi.org/10.1007/s00606-023-01881-z
- Němcová Y, Kreidlová J, Kosová A, Neustupa J (2012) Lakes and Pools of Aquitaine Region (France)–A Biodiversity Hotspot of Synurales in Europe. Nova Hedwigia. Beiheft 95: 1–24. https://doi.org/10.1127/0029-5035/2012/0036
- Nicholls KH (1982) Mallomonas species (Chrysophyceae) from Ontario, Canada including descriptions of two new species. Nova Hedwigia 36: 89–124.
- Nicholls KH (1989) Descriptions of four new Mallomonas taxa (Mallomonadaceae, Chrysophyceae). Journal of Phycology 25: 292–300. https://doi.org/10.1111/j.1529-8817.1989.tb00125.x
- Perty M (1852) Zur Kenntniss kleinster Lebensformen: nach Bau, Funktionen, Systematik, mit Specialverzeichniss der in der Schweiz beobachteten. Jent & Reinert, Bern, 228 pp.
- Pichrtová M, Némcová Y, Škaloud P, Rott E (2013) Silica-scaled chrysophytes from North Tyrol (Austria) including a description of Mallomonas tirolensis sp. nov. Nova Hedwigia. Beiheft 142: 69–85.
- Podunay Y, Martynenko N, Shkurina N, Tran H, Gusev E (2025) Two new Mallomonas species (Synurales, Chrysophyceae) from section Torquatae: Mallomonas sagittifera and Mallomonas trifonovae. Phytotaxa 710(1): 091–104. https://doi.org/10.11646/phytotaxa.710.1.5
- Pruesse E, Peplies J, Glöckner FO (2012) SINA: Accurate high-throughput multiple sequence alignment of ribosomal RNA genes. Bioinformatics (Oxford, England) 28: 1823–1829. https://doi.org/10.1093/bioinformatics/bts252
- Ronquist F, Huelsenbeck JP (2003) MrBayes 3: Bayesian phylogenetic inference under mixed models. Bioinformatics (Oxford, England) 19: 1572–1574. https://doi.org/10.1093/bioinformatics/btg180
- Schmidt-Thomé P, Nguyen H, Pham L, Jarva J, Nuottimäki K (2015) Climate change adaptation measures in Vietnam. Springer International Publishing, Cham, Switzerland, 79–91. https://doi.org/10.1007/978-3-319-12346-2
- Siver PA (1991) The biology of Mallomonas: Morphology, taxonomy and ecology. Kluwer Academic Publishers, Dordrecht, Boston, London, 230 pp. https://doi.org/10.1007/978-94-011-3376-0_5
- Siver PA (1997) The distribution of scaled chrysophytes in the delta region of the Parana River, Argentina. Nova Hedwigia. Beiheft 64(3–4): 421–453. https://doi.org/10.1127/nova.hedwigia/64/1997/421
- Siver PA (2024) Mallomonas gigantica sp. nov. an Eocene synurophyte possessing the largest known siliceous scales. Fottea 24(2): 261–268. https://doi.org/10.5507/fot.2024.006
- Siver PA, Lott AM (2006) Further observations on the scaled Chrysophycean and Synurophycean flora of the Ocala National Forest, Florida, U.S.A. Nordic Journal of Botany 24: 211–233. https://doi.org/10.1111/j.1756-1051.2004.tb00835.x
- Siver PA, Jo BY, Kim JI, Shin W, Lott AM, Wolfe AP (2015) Assessing the evolutionary history of the class Synurophyceae (Heterokonta) using molecular, morphometric, and paleobiological approaches. American Journal of Botany 102(6): 921–941. https://doi.org/10.3732/ajb.1500004
- Škaloud P, Kynčlová A, Benada O, Kofroňová O, Škaloudová M (2012) Toward a revision of the genus Synura, section Petersenianae (Synurophyceae, Heterokontophyta): Morphological characterization of six pseudo-cryptic species. Phycologia 51(3): 303–329. https://doi.org/10.2216/11-20.1
- Škaloud P, Kristiansen J, Škaloudová M (2013) Developments in the taxonomy of silica-scaled chrysophytes — From morphological and ultrastructural to molecular approaches. Nordic Journal of Botany 31: 385–402. https://doi.org/10.1111/j.1756-1051.2013.00119.x
- Škaloud P, Škaloudová M, Jadrná I, Pilátová J, Shin W, Kopecký J (2023) Unravelling the hidden complexity in diversity and pigment composition of a colonial flagellate Synura sphagnicola (Chrysophyceae, Stramenopiles). Fottea, Olomouc 23(2): 149–163. https://doi.org/10.5507/fot.2022.021
- Škaloud P, Škaloudová M, Knotek P, Jadrná I, Pusztai M (2025a) Mallomonas intermedia: A case study of speciation and evolutionary dynamics of protists. Fottea, Olomouc 25(2): 169–182. https://doi.org/10.5507/fot.2025.008
- Škaloud P, Tučková K, Čablová R, Jadrná I, Černajová I (2025b) High-frequency sampling unveils biotic and abiotic drivers of rapid phytoplankton morphological changes. The New Phytologist: 1–14. https://doi.org/10.1111/nph.70534
- Takahashi E (1978) Electron microscopical studies of the Synuraceae (Chrysophyceae) in Japan. Tokai University Press, Tokyo, 194 pp.
- Tran HQ, Mazei YA (2018) Testate amoebae from South Vietnam waterbodies with the description of new species Difflugia vietnamica sp. nov. Acta Protozoologica 57: 215–230. https://doi.org/10.4467/16890027AP.18.016.10092
- Tran HQ, Tran VT, Zagumyonnaya ON, Tikhonenkov DV (2022) Ecology of testate amoebae in waterbodies of the Central Highlands and South-Central Coast provinces of Vietnam with the description of new species Difflugia quangtrani sp. nov. European Journal of Protistology 86: 125933. https://doi.org/10.1016/j.ejop.2022.125933
- Vigna MS, Siver PA (2003) Biodiversity and biogeographical implications of silica-scaled chrysophytes (Chrysophyceae and Synurophyceae) of the northeast wetlands of Argentina. Archiv für Hydrobiologie 158(3): 359–372. https://doi.org/10.1127/0003-9136/2003/0158-0359
- Vyverman W, Cronberg G (1993) Scale bearing chrysophytes from Papua New Guinea. Nordic Journal of Botany 13: 111–120. https://doi.org/10.1111/j.1756-1051.1993.tb00022.x
- Wee JL, Fasone LD, Sattler A, Starks WW, Hurley DL (2001) ITS/5.8S DNA sequence variation in 15 isolates of Synura petersenii Korshikov (Synurophyceae). Nova Hedwigia. Beiheft 122: 245–258.
- Wei Y-X, Yuan X-P (2001) Studies on silica-scaled chrysophytes from the tropics and subtropics of China. Nova Hedwigia. Beiheft 122: 169–187.
- Wujek DE (1984) Scale-bearing Chrysophyceae (Mallomonadaceae) from North-Central Costa Rica. Brenesia 22: 309–313.
- Wujek DE, Dziedzic RM (2005) Silica-scaled chrysophytes from Ecuador. Gayana. Botánica 62(1): 1–8. https://doi.org/10.4067/S0717-66432005000100002
- Wujek DE, Igoe MJ (1989) Studies on Michigan Chrysophyceae. VII. Nova Hedwigia 95: 269–280.
- Wujek DE, Timpano P (1984) The Genus Mallomonopsis in the United States. Transactions of the Kansas Academy of Science 87(3–4): 73–82. https://doi.org/10.2307/3627840