Effect of Deproteinising Agents on The Adhesion of Composite Resin To Dentin in Total Etch and Self Etch Techniques - A Nanoleakage Study
- 1. International Journal of Dental Science and Innovative Research (IJDSIR)
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Abstract
Background: Despite advances in adhesive dentistry, the integrity of the hybrid layer at the dentin–restorative interface is of paramount importance for the long term bond durability of the restoration. Deproteinizing agents such as bromelain and ficin may enhance bonding by removing exposed collagen fibrils, however their effect on nanoleakage with different adhesive systems is not well established.
Aim: The effect of deproteinising agents on the adhesion of composite resin to dentin in total etch and self-etch techniques by evaluating nanoleakage.
Materials and Methods: Sixty extracted human molars were taken and standardized Class V cavities on the buccal surface were prepared. Samples were divided into three groups: Group I - control (no deproteinizing agent), Group II - 10% bromelain, Group III - 2% ficin. Each group was further subdivided into Sub-group A - self-etch and Sub-group B - total-etch adhesive subgroups. Based on the group deproteninzing agents were used and their respective bonding agents were applied. Restorations were completed with nano-hybrid composite resin. Nanoleakage was assessed by assessing rhodamine B dye penetration using confocal laser scanning microscopy.
Results: Bromelain-treated groups demonstrated the least nanoleakage, with a significant reduction under the total-etch technique. Control groups showed moderate leakage, while ficin consistently exhibited the highest nanoleakage values with minimal improvement across etching modes.
Conclusion: Application of deproteinizing agents before bonding significantly reduced nanoleakage. Bromelain showed better results compared to ficin and controls, indicating its potential as an effective deproteinizing agent to enhance dentin bonding durability
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References
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