Published December 17, 2025
| Version v1
Journal
Open
Antibody Characterization Report for Beclin-1 (UniProt ID: Q14457) for use in western blot, immunoprecipitation and flow cytometry
Authors/Creators
- 1. Department of Neurology and Neurosurgery, Structural Genomics Consortium, The Montreal Neurological Institute, McGill University, Montreal, Quebec, Canada
- 2. NIHR BRC-Respiratory, University of Leicester, Leicester, England, UK
Description
Here we have characterized nineteen Beclin-1 commercial antibodies for western blot and immunoprecipitation, and sixteen antibodies for flow cytometry using a standardized experimental protocol based on comparing read-outs in knockout cell lines and isogenic parental controls. This work was supported by the Michael J. Fox Foundation for Parkinson’s Research (MJFF) (grant no. 18331). This work was also supported by a grant from the Quebec Consortium for Drug Discovery (CQDM), a grant from the Ministère de l’Économie, de l’Innovation et de l’Énergie du Québec.