Papiliotrema millettiae C. Y. Chai & F. L. Hui sp. nov.

Fig. 2

Etymology.

The specific epithet millettiae refers to Millettia, the plant genus from which the type strain was isolated.

Typus.

China • Guizhou, Pingtang County, Sifangjing Village, 25°7'N, 107°2'E, on the phylloplane of Millettia pachycarpa, Feb 2024, D. Lu, NYNU 243102 (holotype CICC 33641 preserved in a metabolically inactive state, metabolically inactive ex-type culture PYCC 10057), GenBank Accession No.: PP 837692 (ITS), PP 837690 (LSU).

Description.

On YM agar after 7 days at 20 ° C, the streak culture is white-cream, butyrous and smooth, with an entire margin. After 3 days in YM broth at 20 ° C, cells are ovoid and ellipsoidal, 3.5–6.4 × 4.3–8.9 μm and single, budding is polar. After 1 month at 20 ° C, a ring and a sediment are present. In Dalmau plate culture on CMA, pseudohyphae and hyphae are not formed. Sexual structures are not observed on PDA, CMA or V 8 agar. Ballistoconidia are not produced. Ballistoconidia are not produced. Glucose fermentation is absent. Glucose, inulin (weak and delayed), sucrose, raffinose, galactose, lactose, trehalose, maltose, melezitose, methyl-α-D-glucoside, cellobiose, salicin, L-rhamnose, D-xylose, L-arabinose, D-arabinose, 5 - keto-D-gluconate, D-ribose, ethanol, glycerol, ribitol, galactitol, D-mannitol, D-glucitol, myo-inositol, DL-lactate, succinate, citrate (delayed), D-gluconate, D-glucosamine (weak), N-acetyl-D-glucosamine (weak), 2 - keto-D-gluconate, D-glucuronate and glucono- 1, 5 - lactone are assimilated as carbon sources. Melibiose, L-sorbose, methanol and erythritol are not assimilated. Ethylamine and L-lysine and cadaverine (delayed) are assimilated as nitrogen sources. Nitrate and nitrite are not assimilated. Growth is observed at 20 ° C and 25 ° C, but not at 30 ° C. Growth on 50 % (w / w) glucose-yeast extract agar is positive. Vitamins are not necessary for growth. Starch-like compounds are produced. Diazonium blue B colour and urease reaction are positive.

Additional strain examined.

China, Hainan, Sanya City, Wuzhi Mountain, 32°45'N, 113°30'E, on the phylloplane of Musa nana, Mar 2024, S. L. Lv, NYNU 24472, GenBank Accession No.: PV 823290 (ITS), PV 823291 (LSU).

Note.

P. millettiae is phylogenetically closely related to P. mangalensis, P. pseudoalba, P. hoabinhensis and P. anemochoreius. Physiologically, P. millettiae differs from P. mangalensis in its ability to assimilate inulin, methyl-α-D-glucoside and DL-lactate. Compared to P. pseudoalba, it cannot assimilate melibiose or grow at 30 ° C. It differs from P. hoabinhensis in its ability to assimilate inulin and DL-lactate and to grow on 50 % glucose medium. Compared to P. anemochoreius, it cannot assimilate melibiose or L-sorbose and cannot grow at 30 ° C (Table 2).

Note. 1, P. millettiae; 2, P. mangalensis; 3, P. pseudoalba; 4, P. hoabinhensis; 5, P. anemochoreius; 6, P. mussaendae; 7, P. wisconsinensis. +, positive reaction; –, negative reaction; d, delayed positive; n, not available; s, slow positive; v, variable; w, weakly positive. All data from this study, except * which were obtained from the original description (Boekhout et al. 2011; Fell et al. 2011; Fonseca et al. 2011; Sylvester et al. 2015).