Published December 16, 2025
| Version v1
Journal
Open
Antibody Characterization Report for DJ-1 (PARK7) (UniProt ID: Q99497) for use in western blot, immunoprecipitation, and immunofluorescence
Authors/Creators
- 1. Department of Neurology and Neurosurgery, Structural Genomics Consortium, The Montreal Neurological Institute, McGill University, Montreal, Quebec, Canada
Description
Here we have characterized sixteen DJ-1 commercial antibodies for western blot, immunoprecipitation, and immunofluorescence using a standardized experimental protocol based on comparing read-outs in knockout cell lines and isogenic parental controls. This work was supported by the Michael J. Fox Foundation for Parkinson’s Research (MJFF) (grant no.18331). This work was also supported by a grant from the Quebec Consortium for Drug Discovery (CQDM), a grant from the Ministère de l’Économie, de l’Innovation et de l’Énergie du Québec.