Dataset for: Functional imaging of nine distinct neuronal populations under a miniscope in freely behaving animals

This record contains raw and processed data supporting:
"Functional imaging of nine distinct neuronal populations under a miniscope in freely behaving animals"

Imaging modalities:
- Miniscope calcium imaging (nVoke2)
- In vivo multiplexed confocal spectral imaging (LSM 980)

Folder descriptions:

4FluorophoreData/: Reduced-complexity experiments using four retrograde fluorophores, organized by animal ID.

• Inscopix/: CNMF-E spatial maps and anatomical reference images, organized by imaging day
• ZEISS/: Confocal spectral images and imported ROIs

9FluorophoreData/: Full Neuroplex experiments using nine injected fluorophores (plus GCaMP), organized by animal ID.

• Behavior/: Timestamped behavioral probabilities for each session
• Inscopix/: CNMF-E spatial maps and anatomical reference images, organized by imaging day
• ZEISS/: Confocal spectral images and imported ROIs
• ProcessedData/: ROI definitions, raw and normalized spectral fingerprints, and fluorophore assignments

CalibrationData/: Optical calibration and validation datasets.

• ArgoSlide: Fluorescent calibration slide data used for image-to-image registration and GRIN lens aberration characterization
• Hek293Cells: In vitro spectral imaging data from HEK293T cells expressing individual fluorophores, used to generate pure fluorophore spectral fingerprints

Corresponding analysis code available at:

GitHub - Neurocipher/PythonPipeline: Python pipeline for Neurocipher