Liquid-Suspendable Metabolic Sensors Allow Real-Time Oxygen Consumption Monitoring in Caco-2 and Colon Organoid-Based Gut- on-a-Chip Models

This dataset contains all raw fluorescence and processed oxygen consumption data underlying the figures presented in the manuscript:

“Liquid-Suspendable Metabolic Sensors Allow Real-Time Oxygen Consumption Monitoring in Caco-2 and Colon Organoid-Based Gut-on-a-Chip Models.”

The data were generated using liquid-suspendable metabolic oxygen sensors to monitor time-resolved oxygen consumption in a commercially available microfluidic gut-on-a-chip platform. Measurements were performed in empty chips, Caco-2 epithelial tubules, and adult stem-cell–derived colon organoid models under basal conditions and during mitochondrial stress testing using oligomycin, FCCP, and antimycin A/rotenone.

All datasets required to reproduce Figures 1–4 of the manuscript are provided as Microsoft Excel (.xlsx) files, including:

• Raw fluorescence intensity traces

• Fitted model outputs

• Derived oxygen consumption rates (OCR)

• Mito stress test profiles

• Transepithelial electrical resistance (TEER) data

• Spare respiratory capacity (SRC) calculations

The raw measurements are organized per experiment and per chip unit, with time-stamped fluorescence values and corresponding experimental conditions.

Methods Summary

Fluorescence measurements were acquired at 10-second intervals during static (no-flow) incubation phases in microliter-scale microfluidic channels, followed by re-oxygenation via controlled plate tilting. Oxygen consumption rates were calculated from the first derivative of fitted fluorescence curves. Basal and FCCP phases were fitted using five-parameter logistic (5PL) models; oligomycin and antimycin A/rotenone phases were fitted using linear models.