Published November 20, 2025
| Version v2
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Preprint for: Micropatterning and Nanodropletting of Titanium by Shifted Surface Laser Texturing Significantly Enhances In Vitro Osteogenesis of Healthy and Osteoporotic Mesenchymal Stromal Cells
Authors/Creators
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Stich, Theresia
(Data collector)1
- Alagboso, Francisca (Data collector)1
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Pattappa, Girish
(Data collector)1, 2
- Chu, Jin (Data collector)3
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Moskal, Denys
(Data collector)4
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Povolný, Michal5
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Saller, Maximilian
(Data collector)6
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Schönitzer, Veronika
(Data collector)6
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Scholz, Konstantin J.
(Data collector)7, 8
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Cieplik, Fabian
(Data collector)8
- et al. Show all 15 authors
Authors/Creators
-
Stich, Theresia
(Data collector)1
- Alagboso, Francisca (Data collector)1
-
Pattappa, Girish
(Data collector)1, 2
- Chu, Jin (Data collector)3
-
Moskal, Denys
(Data collector)4
-
Povolný, Michal5
-
Saller, Maximilian
(Data collector)6
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Schönitzer, Veronika
(Data collector)6
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Scholz, Konstantin J.
(Data collector)7, 8
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Cieplik, Fabian
(Data collector)8
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Alt, Volker
(Data curator)1
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Rudert, Maximilian
(Data curator)2
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Kovářík, Tomáš
(Data collector)9
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Křenek, Tomáš
(Data collector)10
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Docheva, Denitsa
(Data curator)1, 2, 4
- 1. Experimental Trauma Surgery, Department of Trauma Surgery, University Regensburg Medical Centre, Regensburg, Germany
- 2. Department of Musculoskeletal Tissue Regeneration and Department of Orthopedics, Orthopedic Hos-pital König-Ludwig-Haus, Julius-Maximilians-University Würzburg, Germany
- 3. Department of Spinal Surgery, The Second Hospital of Dalian Medical University, Dalian Medical University, Dalian, China
- 4. New Technologies Research Centre, University of West Bohemia, Pilsen, Czech Republic
- 5. Department of Machining Technology, Faculty of Mechanical Engineering, University of West Bohemia, Czech Republic
- 6. Department of Orthopedics and Trauma Surgery, Musculoskeletal University Centre Munich (MUM), Ludwig-Maximilians-University (LMU) Hospital, Munich, Germany
- 7. Department of Conservative Dentistry and Periodontology, University Hospital Regensburg, Regens-burg, Germany
- 8. Department of Operative Dentistry and Periodontology, Center for Dental Medicine, Medical Cen-ter—University of Freiburg, Faculty of Medicine, University of Freiburg, Germany
- 9. Západočeská Univerzita v Plzni
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10.
University of West Bohemia in Pilsen
Description
In this experimental paper, titanium (Ti) scaffolds were subjected to shifted laser surface texturing (sLST) using a nanosecond pulsed laser to create an open pore macrotopography with micro-and nano- Ti droplets. In contrast to conventional laser texturing, which leads to high heat accumulation; in sLST, the frequency of laser pulses is low, allowing for resolidification, thereby creating a surface with abundant coverage micro-/nanodroplets. The main objective was to compare the cellular responses of human mesenchymal stromal cells (hMSCs) on sLST-textured Ti surfaces (LT-Ti) for the first time with standard sand-blasted, acid-etched surfaces (SLA-Ti). In-depth analyses of cell survival, proliferation, shape, mineralization, and gene expression were performed.
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Preprint_Stich et al. 2025_main manuscript_submitted to MDPI_watermark.pdf
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Additional details
Identifiers
Related works
- Is cited by
- Journal article: 10.3390/jfb16110401 (DOI)
- Is supplemented by
- Dataset: 10.5281/zenodo.17338495 (DOI)
Funding
- European Union
- Operational Programme Johanes Amos Comenius, call Excellent Research, co funded by the European Union, administered by the Ministry of Education, Sports and Youth CZ.02.01.01/00/22_008/0004634
- European Union
- Project Nr. 349. OSTEOMET: Augmented treatment of osteoporotic bone with innovative organic-inorganic nanoparticle functionalized metal surfaces 349
- European Union
- Project number: BYCZ01-129: KT-Dent: Cross-border transfer of knowledge in the field of advanced dental materials 129