Distinct diet-microbiome associations in autism spectrum disorder

File: Processed_dietary_data.sas7bdat | Description: Dietary metrics used for association analysis.

File: Metaphlan_table.txt | Description: Microbial composition data used for taxonomic profiling.

File: Humann3_pathwayabundance_relab_unstratified.tsv | Description: Microbial pathway abundances used for functional analysis.

File: KO.xlsx | Description: KEGG Orthology  abundances used for functional analysis.

Code.zip - This archive contains all custom R scripts used in the analysis:

• • Variance_explained_plot_and_violin_plot.R: Generates exploratory plots for data visualization.

  • Processed_code_for_diet_microbiome.R: Performs the core diet-microbiome association analysis.

  • Mediation_and_sensitivity_analysis.R: Conducts mediation and sensitivity analyses for microbiome-mediated diet-ASD associations.

  • Food_additive_network_corr.R: Constructs and analyzes microbial co-occurrence networks.

  • Complex_heatmap_in_ASD_and_nonASD file: Code and data for generating the association heatmaps for Figure 2a and Supplementary Figures S2, S3, S4, and S10.