HR-LCMS Analysis for the identification of bioactive compound of Justicia adhatoda L. leaf extract and the potential Antioxidant and Anti-lipid peroxidation Assay In vitro

Throughout human history, medicinal plants have always been used as medicine to treat various diseases, extracts and active compounds of the medicinal plant most use of the therapies and play a major role in medication since the beginning of human civilization and also contribute to the manufacturing of drugs these days. The purpose of this study was to assess the Justicia adhatoda L. butanol (JABE) and methanol (JAME) leaves extracts for potential against lipid peroxidation activity, as well as for several antioxidant assays. A number of characteristics were examined, such as anti-lipid peroxidation, scavenging of free radicals (ferric reducing power potential, iron chelating activity, hydroxyl, hydrogen peroxide, superoxide and nitric oxide radicals). The JABE and JAME extracts was also subjected to HR-LCMS analysis. The HR-LCMS analysis revealed the makeup of a variety of bioactive compounds, such as Andrographolide, Vasicinone, vincamine, Curcumin, Kaempferitrin, 6-Gingerol, Sitagliptin, Kynurenic acid, Oleoyl ethanolamide, Luotonin A and many more, which exhibit numerous pharmacological activities such as antioxidant, anti-inflammatory, anticancer, and antidiabetic, antihypertensive, antimicrobial, antiaging, hepatoprotective, lipid lowering potential. These fractions also demonstrated a strong correlation with the IC₅₀ values for the scavenging of hydrogen peroxide radicals, superoxide radicals, anti-lipid peroxidation, nitric oxide, iron chelating and hydroxyl radical efficacy. Additionally, the maximum scavenging activity for the above mention assays and for the reduction of Fe³⁺ to Fe²⁺ was demonstrated by both the fractions. The current findings indicated that Justicia adhatoda L. leaves, specifically the JABE and JAME fractions, have therapeutic potential as agent to prevent damage caused by free radicals.