supporting files for "Cytokinin down-regulates photosystem II photochemistry during prolonged darkness in a phytochrome B-dependent manner"

Fig.1_source data. Source data for panel A of Fig.1: chlorophyll a+b content and chlorophyl a/b ratio in Arabidopsis Col-0 rosette leaves of 33-days old plants that were detached and treated with mock (0.2% DMSO), BAP or MTU at the concentration of 0.5 or 5 µM, and kept for 2, 5, 7, 9 or 12 days in the dark (days after detachment and darkening, dad).

Fig.2_source data. Source data for each panel of Fig.2: (A) Maximum quantum yield of PSII photochemistry (F_V/F_M). (B) Quantum yields of PSII photochemistry (Φ_P), regulatory non-photochemical quenching (Φ_NPQ), and non-regulatory dissipation processes (Φ_f,D). (C) Electron transport rates through PSII (ETRII), PSI (ETRI), and cyclic electron transport (CET). (D) ROS accumulation visualized using 2′,7′-dichlorodihydrofluorescein diacetate (DCF-DA) and quantified from mean green-fluorescence intensity. (E) F_V/F_M in cytokinin receptor double mutants and selected cytokinin response-regulator mutants. Detached leaves were incubated with mock (0.2% DMSO) or 5 µM BAP in darkness for 2 days. (F) F_V/F_M in cytokinin receptor double mutants and selected cytokinin response-regulator mutants. Detached leaves were incubated with mock (0.2% DMSO) or 5 µM MTU in darkness for 2 days.

Fig. 7_source data. Source data for each panel of Fig.7: (A) Maximum quantum yield of PSII photochemistry (F_V/F_M), (B) cyclic electron transport (CET), and (C) malondialdehyde (MDA) content, in detached leaves of WT, phytochrome-deficient mutants (phyA or phyB), and phytochrome B overexpressing line (phyB OE).

Fig. 8_source data. Source data for panel A: Maximum quantum yield of PSII photochemistry (F_V/F_M) in hydroponically grown Arabidopsis WT or phyB mutant plants after 1 or 3 days in the dark. Plants were treated with mock (0.02 % DMSO), BAP or MTU at the concentration of 5 or 10 µM.

Fig. S1_source data. Source data for Fig. S1: Maximum quantum yield of PSII photochemistry (F_V/F_M) in selected time points. Leaves from 33-day-old Arabidopsis WT plants (Col-0) were detached and incubated in the dark while submerged in solutions of 0.2% DMSO, 5 μM BAP, or 5 μM MTU for 2 days, and afterwards the F_V/F_M was measured (time point 1). The leaves were subsequently re-illuminated by returning them to the standard growing conditions (8-h light (110 µmol photons m-2 s-1; LED)/16-h dark cycle, and at 22/20 °C), and before the end of the photoperiod, F_V/F_M was measured again (time point 2). On the following day, approximately 3 h after the start of the photoperiod, the F_V/F_M was measured for the last time (time point 3).