MARCO-BOLO - Metadata V9-18S rDNA amplicon sequencing of NEREA environmental DNA samples

Grant Agreement: 101082021
Project Acronym: MARCO-BOLO
Project Title: MARine COastal BiOdiversity Long-term Observations
Deliverable Number: D2.2
Work Package Number: WP2
Deliverable Title: Datasets, databases and softwares/pipelines facilitating the implementation of eDNA-based monitoring

Analysis of the V9 region of 18S rDNA in the environmental DNA from seawater through amplicon sequencing. Samples were collected monthly between September 2021 and December  2023, across different sampling sites within the context of the NEREA observatory in the Gulf of Naples (Italy). Seawater was collected at discrete depths, filtered through 0.45 micron filters, and the environmental DNA was extracted from and amplified by PCR. Plankton metabarcoding raw reads were registered in the European Nucleotide Archive (ENA), under the umbrella project permanent identifier PRJEB74649 (https://identifiers.org/ena.embl:PRJEB74649). The raw eukaryotic metabarcoding sequencing data files - V9-18S (FastQ format) are available on BioProject ID PRJEB98195.