Prediction and mapping of promiscuous MHC class II binders in an antigen sequence

There is tremendous change in strategy used for developing vaccine, over the years from whole pathogen to antigens and antigens to antigenic regions (epitopes). In subunit vaccine epitope is used instead of a complete protein as vaccine candidate. Thus prediction of epitopes particularly T-help epitopes is one of the major challenges in subunit vaccine design. It is well established that binding of a peptide to an MHC Class II molecule is a prerequisite for activation of antigen specific T-helper cells. In past number of methods have been developed for predicting MHC class II binders but these methods allow to predict binder for one or two MHC alleles. Thus not suitable for wide population as individual have limited number of MHC alleles. In order to overcome this problem we developed a method for predicting promiscuous MHC binders, which binds two or more than two alleles. This server will be useful for designing subunit vaccine effective for wide population.