Published November 20, 2025 | Version v1

Deep Learning Virtual Staining Dataset: Breast, Mouse, and Organoids

  • 1. ROR icon Beihang University
  • 2. ROR icon Chinese PLA General Hospital

Description

This dataset contains comprehensive image data for training and evaluating deep learning models dedicated to virtual staining of histopathological images. The goal is to generate H&E-like pathology images from autofluorescence (AF) or multi-channel fluorescence images. It covers multiple tissue types including human breast tissue, mouse tumors, and organoids, facilitating research in label-free imaging and computational pathology.

Most processed images (patches and evaluation sets) are in standard TIF format.  The files inside Initial_data.zip are in 3DHISTECH Mirax proprietary format (.mrxs). To open MRXS files: Please use 3DHISTECH SlideViewer (formerly CaseViewer) or the open-source software QuPath.

Files

Breast_Sub_eval.zip

Files (27.0 GB)

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