Published November 15, 2025 | Version v1
Journal article Open

ISOLATION, BIOCHEMICAL CHARACTERISATION AND MULTI DRUG POTENTIAL OF URINARY TRACT INFECTIOUS BACTERIA

Authors/Creators

  • 1. Department of Life Sciences, Hindu College – Guntur.

Description

One of the most common infectious diseases, urinary tract infections (UTIs) impact millions of people worldwide, especially women and those with weakened immune systems. Numerous bacteria that colonise the urinary system and cause inflammation, discomfort, and other symptoms are the main cause of UTI pathogenesis. Following Escherichia coli as the most commonly isolated pathogen are other Gram-negative bacteria, including Pseudomonas aeruginosa, Klebsiella spp., and Proteus spp. In order to ascertain the therapeutic potential of the bacterial pathogens that cause UTIs, this study will isolate and identify them, describe their virulence characteristics, and assess their antibiotic susceptibility profiles. Collected and processed three patient urine samples. Selective media were used for bacterial isolation, and Gramme staining, biochemical testing, and antibiotic susceptibility testing were used for identification. High sensitivity rates to widely used antibiotics were found through antibiotic susceptibility testing utilising the Kirby-Bauer disc diffusion method. Interestingly, a large number of strains showed multidrug sensitivity.

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