DNA preservation on blotting paper & DNA extraction protocol for field collection of coral samples suitable for marker gene sequencing approaches

Summary

Collecting biological samples from remote field settings presents logistical challenges in preserving and transporting DNA to laboratory facilities for further processing (e.g., DNA extraction, PCR amplification, and sequencing). To address this, we tested preserving DNA samples on blotting paper, offering a practical and efficient solution for transporting biological samples as a dry medium. Blotting papers, commonly used for the transfer and immobilization of nucleic acids and proteins, have high binding affinity and absorption capacity, making them suitable for DNA preservation. Coral samples, in the form of sprayed off tissue in DESS buffer, are pipetted onto blotting paper for storage and transport. Using the Qiagen Dneasy Plant Pro Kit that entails a bead-beating step to effectively lyse the samples, we obtained sufficient DNA for subsequent marker gene sequencing approaches. This method enhances the feasibility of conducting genetic research in remote locations by overcoming the limitations associated with liquid sample preservation and transportation.

Funding

MS and CRV are supported by the German Research Foundation (DFG), project: 468583787. KLC, SS, CRV are supported by G20 Coral Research & Development Accelerator Platform (CORDAP), Coral Accelerator Program 2022 (CAP), Project ASSIST, grant: CAP-2022-1591.