Published November 2025
| Version v1
Dataset
Open
In vitro integration site sequencing for "Core nucleosomes are refractory to lentiviral DNA integration: the role of cellular LEDGF/p75 protein"
Authors/Creators
-
1.
University of Oxford
-
2.
The Francis Crick Institute
-
3.
Dana-Farber Cancer Institute
- 4. Imperial College London
Description
Long-read Oxford Nanopore Technologies native sequencing of human immunodeficiency virus (HIV-1) and prototype foamy virus (PFV) integration of purified in vitro assembelled intasomes integrating into in vitro assembelled poly-nucleosomal arrays containing 12 repeats of the Widom 601 nucleosome positioning sequence and a 50 bp linker. Nucleosomes were formed of unmodified histone octamers or octamers bearing histone H3 trimethylated on lysine-36 by native chemical ligation.
Replicates and metadata for each sample are represented in full in the metadata.tsv file.
Indicated experiments were doped with naked phiX174 double-stranded DNA.
Files
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Additional details
Funding
- National Institutes of Health
- U54AI170791
- National Institutes of Health
- R37AI039394
- The Francis Crick Institute
- Cancer Research UK
- CC2058
- Medical Research Council
- CC2058
- Wellcome Trust
- CC2058