Published November 3, 2025 | Version v1

New species of Aspergillus in sections Cavernicolarum and Nigri from terrestrial ecosystems of China (Eurotiales, Aspergillaceae)

  • 1. Institute of Microbiology, Chinese Academy of Sciences, Beijing, China|University of Chinese Academy of Sciences, Beijing, China
  • 2. Institute of Microbiology, Chinese Academy of Sciences, Beijing, China

Description

Aspergillus species are of great industrial, agricultural, and medicinal importance. During investigations on the biodiversity of Aspergillaceae, two species of Aspergillus isolated from soil samples in China were identified as new to science based on sequence analyses and morphological comparisons. Aspergillus hebeiensis from a traditional cultural and Buddhist heritage site is the second member of the series Hainanici in section Cavernicolarum of subgenus Nidulantes, while A. xishuangbannaensis from a tropical nature reserve is classified in subgenus Circumdati, section Nigri, series Japonici. Detailed descriptions and illustrations of both species are provided, and the ecological functions of their habitats are also discussed.

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References

  • Alves VCS, Lira RA, Lima JMS, Barbosa RN, Bento DM, Barbier E, Bernard E, Souza-Motta CM, Bezerra JDP (2022) Unravelling the fungal darkness in a tropical cave: Richness and the description of one new genus and six new species. Fungal Systematics and Evolution 10: 139–167. https://doi.org/10.3114/fuse.2022.10.06
  • Bennett JW (2009) Aspergillus: A primer for the novice. Medical Mycology 47(Suppl 1): S5–S12. https://doi.org/10.1080/13693780802712515
  • Chen K, Zhuang WY (2017) Discovery from a large-scaled survey of Trichoderma in soil of China. Scientific Reports 7: 9090. https://doi.org/10.1038/s41598-017-07807-3
  • Crous PW, Catcheside DEA, Catcheside PS, Alfenas AC, Alfenas RF, Barreto RW, Lebel T, Balashov S, Broadbridge J, Jurjevic Z, De la Pena-Lastra S, Hoffmann R, Mateos A, Riebesehl J, Shivas RG, Soliz Santander FF, Tan YP, Altes A, Bandini D, Carriconde F, Cazabonne J, Czachura P, Gryta H, Eyssartier G, Larsson E, Pereira OL, Rigueiro-Rodriguez A, Wingfield MJ, Ahmad W, Bibi S, Denman S, Esteve-Raventos F, Hussain S, Illescas T, Luangsa-Ard JJ, Moller L, Mombert A, Noisripoom W, Olariaga I, Pancorbo F, Paz A, Piatek M, Polman-Short C, Suarez E, Afshan NS, Ali H, Arzanlou M, Ayer F, Barratt J, Bellanger JM, Bidaud A, Bishop-Hurley SL, Bohm M, Bose T, Campo E, Chau NB, Colak OF, Cordeiro TRL, Cruz MO, Custodio FA, Couceiro A, Darmostuk V, Dearnaley JDW, de Azevedo Santiag A, de Freitas LWS, Yanez-Morales MJ, Domnauer C, Dentinger B, Dhileepan K, De Souza JT, Dovana F, Eberhardt U, Eisvand P, Erhard A, Fachada V, Garcia-Martin A, Groenewald M, Hammerbacher A, Harms K, Haroon S, Haqnawaz M, Henriques S, Hernandez AJ, Jacobus LM, Jaen-Contreras D, Jangsantear P, Kaygusuz O, Knoppersen R, Kumar TKA, Lynch MJ, Mahiques R, Maraia GL, Marbach PAS, Mehrabi-Koushki M, Miller PR, Mongkolsamrit S, Moreau PA, Oberlies NH, Oliveira JA, Orlovich D, Perez-Mendez AS, Pinto A, Raja HA, Ramirez GH, Raphael B, Rodrigues A, Rodrigues H, Ramos DO, Safi A, Sarwar S, Saar I, Sanchez RM, Santana JS, Scrace J, Sales LS, Silva LNP, Stryjak-Bogacka M, Tacconi A, Thanh VN, Thomas A, Thuy NT, Toome M, Valdez-Carrazco JM, van Vuuren NI, Vasey J, Vauras J, Vila-Vicosa C, Villarreal M, Visagie CM, Vizzini A, Whiteside EJ, Groenewald JZ (2025) Fungal Planet description sheets: 1781–1866. Persoonia 54: 327–587. https://doi.org/10.3114/persoonia.2025.54.10
  • Fan XY, Matsumoto H, Xu HR, Fang HD, Pan QQ, Lv TX, Zhan CF, Feng XX, Liu XY, Su DR, Fan MY, Ma ZH, Berg G, Li SJ, Cernava T, Wang MC (2024) Aspergillus cvjetkovicii protects against phytopathogens through interspecies chemical signalling in the phyllosphere. Nature Microbiology 9: 2862–2876. https://doi.org/10.1038/s41564-024-01781-z
  • Frisvad JC, Larsen TO, de Vries R, Meijer M, Houbraken J, Cabanes FJ, Ehrlich K, Samson RA (2007) Secondary metabolite profiling, growth profiles and other tools for species recognition and important Aspergillus mycotoxins. Studies in Mycology 59: 31–37. https://doi.org/10.3114/sim.2007.59.04
  • Glass NL, Donaldson GC (1995) Development of primer sets designed for use with the PCR to amplify conserved genes from filamentous ascomycetes. Applied and Environmental Microbiology 61: 1323–1330. https://doi.org/10.1128/aem.61.4.1323-1330.1995
  • Hall TA (1999) BioEdit: A user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucleic Acids Symposium Series 41: 95–98.
  • Hong SB, Go SJ, Shin HD, Frisvad JC, Samson RA (2005) Polyphasic taxonomy of Aspergillus fumigatus and related species. Mycologia 97: 1316–1329. https://doi.org/10.3852/mycologia.97.6.1316
  • Houbraken J, Kocsube S, Visagie CM, Yilmaz N, Wang XC, Meijer M, Kraak B, Hubka V, Bensch K, Samson RA, Frisvad JC (2020) Classification of Aspergillus, Penicillium, Talaromyces and related genera (Eurotiales): An overview of families, genera, subgenera, sections, series and species. Studies in Mycology 95: 5–169. https://doi.org/10.1016/j.simyco.2020.05.002
  • Huang L, Tian L, Huang L, Jin C, Hu S, Zhang Z, Yan E, Jim CY, Yang Y, Lindenmayer DB, Tang Z (2025) Religious temples are long-term refuges for old trees in human-dominated landscapes in China. Current Biology 35: 2994–3000. https://doi.org/10.1016/j.cub.2025.05.030
  • Katoh K, Standley DM (2013) MAFFT multiple sequence alignment software version 7: Improvements in performance and usability. Molecular Biology and Evolution 30: 772–780. https://doi.org/10.1093/molbev/mst010
  • Khan S, Bilal H, Shafiq M, Zhang D, Awais M, Chen C, Khan MN, Wang Q, Cai L, Islam R, Zeng Y (2024) Distribution of Aspergillus species and risk factors for aspergillosis in mainland China: A systematic review. Therapeutic Advances in Infectious Disease 11: 20499361241252537. https://doi.org/10.1177/20499361241252537
  • Klich MA (2002) Identification of common Aspergillus species. Centraalbureau voor Schimmelcultures, Utrecht, The Netherlands, 116 pp.
  • Lima JMS, Barbosa RN, Bento DM, Barbier E, Bernard E, Bezerra JDP, Souza-Motta CM (2024) Aspergillus, Penicillium, and Talaromyces (Eurotiales) in Brazilian caves, with the description of four new species. Fungal Systematics and Evolution 14: 89–107. https://doi.org/10.3114/fuse.2024.14.06
  • Liu YJ, Whelen S, Hall BD (1999) Phylogenetic relationships among ascomycetes: Evidence from an RNA polymerse II subunit. Molecular Biology and Evolution 16: 1799–1808. https://doi.org/10.1093/oxfordjournals.molbev.a026092
  • Liu C, Wang XC, Yu ZH, Zhuang WY, Zeng ZQ (2023) Seven new species of Eurotiales (Ascomycota) isolated from tidal flat sediments in China. Journal of Fungi (Basel, Switzerland) 9: 960. https://doi.org/10.3390/jof9100960
  • Mowafy AM (2020) Biological leaching of rare earth elements. World Journal of Microbiology & Biotechnology 36: 61. https://doi.org/10.1007/s11274-020-02838-x
  • Peng LY, Wang XC, Gafforov Y, Zhuang WY (2025) Seven new series and four new species in sections Subinflati and Trachyspermi of Talaromyces (Trichocomaceae, Eurotiales). Journal of Fungi (Basel, Switzerland) 11: 508. https://doi.org/10.3390/jof11070508
  • Perrone G, Varga J, Susca A, Frisvad JC, Stea G, Kocsube S, Toth B, Kozakiewicz Z, Samson RA (2008) Aspergillus uvarum sp. nov., an uniseriate black Aspergillus species isolated from grapes in Europe. International Journal of Systematic and Evolutionary Microbiology 58: 1032–1039. https://doi.org/10.1099/ijs.0.65463-0
  • Posada D, Crandall KA (1998) MODELTEST: Testing the model of DNA substitution. Bioinformatics (Oxford, England) 14: 817–818. https://doi.org/10.1093/bioinformatics/14.9.817
  • Ronquist F, Teslenko M, van der Mark P, Ayres DL, Darling A, Hohna S, Larget B, Liu L, Suchard MA, Huelsenbeck JP (2012) MrBayes 3.2: Efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology 61: 539–542. https://doi.org/10.1093/sysbio/sys029
  • Samson RA, Noonim P, Meijer M, Houbraken J, Frisvad JC, Varga J (2007) Diagnostic tools to identify black aspergilli. Studies in Mycology 59: 129–145. https://doi.org/10.3114/sim.2007.59.13
  • Samson RA, Visagie CM, Houbraken J, Hong SB, Hubka V, Klaassen CH, Perrone G, Seifert KA, Susca A, Tanney JB, Varga J, Kocsube S, Szigeti G, Yaguchi T, Frisvad JC (2014) Phylogeny, identification and nomenclature of the genus Aspergillus. Studies in Mycology 78: 141–173. https://doi.org/10.1016/j.simyco.2014.07.004
  • Schuster E, Dunn-Coleman N, Frisvad JC, Van Dijck PW (2002) On the safety of Aspergillus niger – a review. Applied Microbiology and Biotechnology 59: 426–435. https://doi.org/10.1007/s00253-002-1032-6
  • Tamura K, Stecher G, Kumar S (2021) MEGA11: Molecular Evolutionary Genetics Analysis Version 11. Molecular Biology and Evolution 38: 3022–3027. https://doi.org/10.1093/molbev/msab120
  • Trifinopoulos J, Nguyen LT, von Haeseler A, Minh BQ (2016) W-IQ-TREE: A fast online phylogenetic tool for maximum likelihood analysis. Nucleic Acids Research 44: W232–235. https://doi.org/10.1093/nar/gkw256
  • Varga J, Frisvad JC, Kocsube S, Brankovics B, Toth B, Szigeti G, Samson RA (2011) New and revisited species in Aspergillus section Nigri. Studies in Mycology 69: 1–17. https://doi.org/10.3114/sim.2011.69.01
  • Visagie CM, Goodwell M, Nkwe DO (2021) Aspergillus diversity from the Gcwihaba Cave in Botswana and description of one new species. Fungal Systematics and Evolution 8: 81–89. https://doi.org/10.3114/fuse.2021.08.07
  • Visagie CM, Yilmaz N, Kocsubé S, Frisvad JC, Hubka V, Samson RA, Houbraken J (2024) A review of recently introduced Aspergillus, Penicillium, Talaromyces and other Eurotiales species. Studies in Mycology 107: 1–66. https://doi.org/10.3114/sim.2024.107.01
  • Wang XC, Zhuang WY (2022) New species of Aspergillus (Aspergillaceae) from tropical islands of China. Journal of Fungi (Basel, Switzerland) 8: 225. https://doi.org/10.3390/jof8030225
  • Wang X, Jin C, Huang L, Zhou L, Zheng M, Qian S, Yang Y (2020) Plant diversity and species replacement in Chinese Buddhist temples. Shengwu Duoyangxing 28: 668–677. https://doi.org/10.17520/biods.2019392
  • Wang XC, Zhang ZK, Zhuang WY (2023) Species diversity of Penicillium in Southwest China with discovery of forty-three new species. Journal of Fungi (Basel, Switzerland) 9: 1150.
  • Wei S, Xu X, Wang L (2021) Four new species of Talaromyces section Talaromyces discovered in China. Mycologia 113: 492–508. https://doi.org/10.1080/00275514.2020.1853457
  • White TJ, Bruns TD, Lee SB, Taylor JW (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (Eds) PCR Protocols: a guide to methods and applications. Academic Press, New York, USA, 315–322. https://doi.org/10.1016/B978-0-12-372180-8.50042-1
  • Wu WP, Diao YZ (2022) Anamorphic chaetosphaeriaceous fungi from China. Fungal Diversity 116: 1–546. https://doi.org/10.1007/s13225-022-00509-w
  • Xu Y, Huang H, Lu H, Wu M, Lin M, Zhang C, Zhao Z, Li W, Zhang C, Li X, Sun B (2022) Characterization of an Aspergillus niger for efficient fatty acid ethyl ester synthesis in aqueous phase and the molecular mechanism. Frontiers in Microbiology 12: 820380. https://doi.org/10.3389/fmicb.2021.820380
  • Xue M, Qu Z, Moretti A, Logrieco AF, Chu H, Zhang Q, Sun C, Ren X, Cui L, Chen Q, An Y, Li C, Zhong H, Cao Z, Wang F, Sun Y, Wang L, Hou J, Zhang C, Yang M, Ding Y, Yao Y, Li P, Zhu YG (2025) Aspergillus mycotoxins: The major food contaminants. Advanced Science (Weinheim, Baden-Württemberg, Germany) 12: e2412757. https://doi.org/10.1002/advs.202412757
  • Zhang ZF, Zhou SY, Eurwilaichitr L, Ingsriswang S, Raza M, Chen Q, Zhao P, Liu F, Cai L (2021) Culturable mycobiota from Karst caves in China II, with descriptions of 33 new species. Fungal Diversity 106: 29–136. https://doi.org/10.1007/s13225-020-00453-7