Greenase: A Novel, Comprehensive Carbon Fixation Enzyme Database for Information Retrieval in Carbon Fixation

Greenase is a comprehensive biological information resource designed to centrally collect, organize, and provide detailed information on key enzymes involved in biological carbon fixation processes. The database compiles data on carbon-fixing enzymes—such as enzyme names, substrates, products, sources, activity, Michaelis constants (Km), catalytic efficiency (Kcat/Km), optimal temperature, pH values, and amino acid sequences—from open-source databases and literature. After preliminary screening and curation, it constructs a comprehensive source dataset.

Developed as a Java-based web application using the Spring Boot + MyBatis framework, Vue.js and Element UI for the frontend, and MySQL for the database, Greenase offers a user-friendly and feature-rich platform. It supports efficient searches based on criteria such as "Product," "Substrate," "Enzyme name," and "EC number." Additionally, the database provides atomic economy calculations and data visualization tools, helping users intuitively understand key aspects of carbon-fixing enzymes, including their distribution, origin, and economic efficiency.

Greenase additionally incorporates integrated tools for protein structure prediction and clustering analysis, enabling users to conduct in depth studies of carbon fixation enzymes. These functionalities facilitate the exploration of enzyme 3D conformations and the identification of structural relationships within enzyme families, thus supporting advanced research on carbon fixation enzyme .