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Published October 29, 2025 | Version v1
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Unexpected species richness of the lichen genus Protoblastenia (Lecanorales, Psoraceae) in Finland

Authors/Creators

  • 1. Nature Solutions, Finnish Environmental Institute, Helsinki, Finland
  • 2. University of Helsinki, Helsinki, Finland

Description

The taxonomy of Protoblastenia in Finland was studied, based on morphology and molecular data (nuITS rDNA sequences). Twenty species were recognised, with sixteen species being newly described here: P. arupii sp. nov., P. borealis sp. nov., P. compressa sp. nov., P. dolomitica sp. nov., P. ekmanii sp. nov., P. fennoarctica sp. nov., P. minuta sp. nov., P. oulankaensis sp. nov., P. pseudocompressa sp. nov., P. pseudoterricola sp. nov., P. remota sp. nov., P. rikkinenii sp. nov., P. saanaensis sp. nov., P. timdalii sp. nov., P. violacea sp. nov. and P. westbergii sp. nov. All species are confined to calcareous rocks, except P. terricola which also grows on calcareous soil. The calcareous fells in Enontekiö in NW Finland were identified as hot spots of Protoblastenia diversity. Nine newly-described species (P. arupii, P. fennoarctica, P. minuta, P. pseudoterricola, P. rikkinenii, P. saanaensis, P. timdalii, P. violacea and P. westbergii) are restricted to this area in Finland. Several of the species are semi-cryptic. On average, they may have minor morphological differences, but many specimens cannot be identified, based on morphology only. Protoblastenia rikkinenii is reported from Norway and P. oulankaensis from Italy, based on GenBank sequences. Full descriptions and a preliminary key of Protoblastenia in Finland are provided.

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Cites
Publication: 10.1111/boj.12358 (DOI)
Publication: 10.1093/nar/gkh340 (DOI)
Publication: 10.1093/sysbio/syr022 (DOI)
Publication: 10.1007/s11557-025-02045-8 (DOI)
Publication: 10.1111/j.1365-294X.1993.tb00005.x (DOI)
Publication: 10.1186/s43008-020-00033-z (DOI)
Publication: 10.35535/pfsyst-2020-0025 (DOI)
Publication: 10.1016/j.ympev.2014.04.003 (DOI)
Publication: 10.3398/064.082.0201 (DOI)
Publication: 10.1017/S0024282911000132 (DOI)
Publication: 10.1017/S0024282911000867 (DOI)
Publication: 10.35535/pfsyst-2023-0011 (DOI)
Publication: 10.1017/S0024282916000542 (DOI)
Publication: 10.3897/mycokeys.72.56223 (DOI)
Publication: 10.1093/bioinformatics/btu033 (DOI)
Publication: 10.1016/B978-0-12-372180-8.50042-1 (DOI)
Publication: 10.3390/jof8050490 (DOI)
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Figure: 10.3897/mycokeys.124.162802.figure1 (DOI)

References

  • Cannon P, Aptroot A, Coppins B, Orange A, Sanderson N, Simkin J (2022) Lecanorales: Psoraceae, including the genera Brianaria, Protoblastenia, Protomicarea and Psora. Revisions of British and Irish Lichens 28: 1–11.
  • Castello M, Nimis PL (1995) A critical revision of Antarctic lichens described by C. W. Dodge. Bibliotheca Lichenologica 57: 71–92.
  • Divakar PK, Leavitt SD, Molina MC, Del-Prado R, Lumbsch HT, Crespo A (2016) A DNA barcoding approach for identification of hidden diversity in Parmeliaceae (Ascomycota): Parmelia sensu stricto as a case study. Botanical Journal of the Linnean Society 180: 21–29. https://doi.org/10.1111/boj.12358
  • Edgar RC (2004) MUSCLE: Multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Research 32: 1792–1797. https://doi.org/10.1093/nar/gkh340
  • Ekman S, Blaalid R (2011) The devil in the details: Interactions between the branch-length prior and likelihood model affect node support and branch lengths in the phylogeny of the Psoraceae. Systematic Botany 60: 541–561. https://doi.org/10.1093/sysbio/syr022
  • Evankow AM, Yin A, Zulfiqar R, Ahmad UF, Nordenhaug P, Khalid AN, Wang L, Timdal E (2025) Psora mediterranea (Lecanorales, Psoraceae), a new lichen species from Europe, including a new concept for P. himalayana and a revised key to the European species. Mycological Progress 24: 26. https://doi.org/10.1007/s11557-025-02045-8
  • Gardes M, Bruns TD (1993) ITS primers with enhanced specificity for basidiomycetes – application to the identification of mycorrhizae and rusts. Molecular Ecology 2: 113–118. https://doi.org/10.1111/j.1365-294X.1993.tb00005.x
  • Hafellner J (2006) Protoblastenia szaferi (lichenized Ascomycotina) – new to the Alps. Herzogia 19: 23–33.
  • Kainz C (2004) Protoblastenia. In: Nash III TH, Ryan BD, Diederich P, Gries C, Bungartz F (Eds) Lichen Flora of the Greater Sonoran Desert Region. Volume II. Lichens Unlimited, Arizona State University, Tempe, 424–425.
  • Kainz C, Rambold G (2004) A phylogenetic study of the lichen genus Protoblastenia (Lecanorales, Psoraceae) in Central Europe. Bibliotheca Lichenologica 88: 267–299.
  • Lücking R, Aime MC, Robbertse B, Miller AN, Ariyawansa HA, Aoki T, Cardinali G, Crous PW, Druzhinina IS, Geiser DM, Hawksworth DL, Hyde KD, Irinyi L, Jeewon R, Johnston PR, Kirk PM, Malosso E, May TW, Meyer W, Öpik M, Robert V, Stadler M, Thines M, Vu D, Yurkov ANM, Zhang N, Schoch CL (2020a) Unambiguous identification of fungi: Where do we stand and how accurate and precise is fungal DNA barcoding? IMA Fungus 11: 1–32. https://doi.org/10.1186/s43008-020-00033-z
  • Lücking R, Nadel MRA, Araujo E, Gerlach A (2020b) Two decades of DNA barcoding in the genus Usnea (Parmeliaceae): How useful and reliable is the ITS? Plant and Fungal Systematics 65: 303–357. https://doi.org/10.35535/pfsyst-2020-0025
  • Miadlikowska J, Kauff H, Högnabba F, Oliver JC, Molnár K, Fraker E, Gaya E, Hafellner J, Hofstetter V, Gueidan C, Otálora MAG, Hodkinson B, Kukwa M, Lücking R, Björk C, Sipman HJM, Burgaz AR, Thell A, Passo A, Myllys L, Goward T, Fernández-Brime S, Hestmark G, Lendemer J, Lumbsch HT, Schmull M, Schoch CL, Sérusiaux E, Maddison DR, Arnold AE, Lutzoni F, Stenroos S (2014) A multigene phylogenetic synthesis for the class Lecanoromycetes (Ascomycota): 1307 fungi representing 1139 infrageneric taxa, 317 genera and 66 families. Molecular Phylogenetics and Evolution 79: 132–168. https://doi.org/10.1016/j.ympev.2014.04.003
  • Munger I, Baugh M, Henrie JR, Hollinger J, Crepeau R, Leavitt SD (2022) Integrative biodiversity inventories: Characterizing lichen-forming fungal diversity in Glen Canyon National Recreation Area using DNA barcoding and vouchered specimens. Western North American Naturalist 82(2): 213–233. https://doi.org/10.3398/064.082.0201
  • Myllys L, Velmala S, Holien H, Halonen P, Wang LS, Goward T (2011) Phylogeny of the genus Bryoria. Lichenologist (London, England) 45: 617–638. https://doi.org/10.1017/S0024282911000132
  • Nowak J (1974) Protoblastenia szaferi sp. nov., a new lichen species in the calcareous part of the Polish Tatra Mts. Fragmenta Floristica et Geobotanica 20: 529–533.
  • Orange A (2012) Semi-cryptic marine species of Hydropunctaria (Verrucariaceae, lichenized Ascomycota) from north-west Europe. Lichenologist (London, England) 44: 299–320. https://doi.org/10.1017/S0024282911000867
  • Poelt J (1957) Mitteleuropäische Flechten. V. Mitteilungen der Botanischen Staatssammlung München 17–18: 386–399.
  • Printzen C, Holien H, Kantelinen A, Myllys L, Ratschow F, Stepanchikova I, Weber L, Timdal E (2023) DNA barcoding indicates the presence of unrecognized species and phylogenetic diversity within the Biatora vernalis-and B. meiocarpa-groups. Plant and Fungal Systematics 68: 262–279. https://doi.org/10.35535/pfsyst-2023-0011
  • Pykälä J (2010) Notes on the lichen flora of Saana and Malla fells in northern Finland. Memoranda Societatis pro Fauna et Flora Fennica 86: 34–42.
  • Pykälä J (2023) Additions to the lichen flora of Finland. X. Graphis Scripta 35(3): 14–29.
  • Pykälä J, Lommi S (2021) Lichen flora of Finland – short history of Finnish lichenology and updated species statistics. Memoranda Societatis pro Fauna et Flora Fennica 97: 73–88.
  • Pykälä J, Launis A, Myllys L (2017) Four new species of Verrucaria from calcareous rocks in Finland Lichenologist 49: 27–37. https://doi.org/10.1017/S0024282916000542
  • Pykälä J, Kantelinen A, Myllys L (2020) Taxonomy of Verrucaria species characterised by large spores, perithecia leaving pits in the rock and a pale thin thallus in Finland. MycoKeys 72: 43–92. https://doi.org/10.3897/mycokeys.72.56223
  • Stamatakis A (2014) RAxML version 8: A tool for phylogenetic analysis and post-analysis of large phylogenies. Bioinformatics (Oxford, England) 30(9): 1312–1313. https://doi.org/10.1093/bioinformatics/btu033
  • Stenroos S, Velmala S, Pykälä J, Ahti T [Eds] (2016) Lichens of Finland. Norrlinia 30: 1–896.
  • Svensson M, Ekman S, Arup U, Eide Ekman L, Hammarström O, Isaksson R, Jonsson F, Palice Z, Vicente R, Westberg M (2024) Further additions to the Swedish flora of lichenised fungi. Graphis Scripta 36(2): 15–49.
  • Timdal E (1987) Problems of generic delimitation among squamiform members of the Lecideaceae. In: Peveling E (Ed.) Progress and problems in lichenology in the eighties. Bibliotheca Lichenologica 25: 243–247.
  • White TJ, Bruns T, Lee S, Taylor J (1990) Amplification and direct sequencing of fungal ribosomal DNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (Eds) PCR Protocols: a guide to methods and applications. Academic Press, San Diego, 315–322. https://doi.org/10.1016/B978-0-12-372180-8.50042-1
  • Wirth V, Hauck M, Schultz M (2013) Die Flechten Deutschlands. Band 2. Ulmer, Stuttgart, 1244 pp.
  • Zahlbruckner A (1936) Neue Flechten. XII. Annales Mycologici 34: 159–179.
  • Zhang Y, Clancy J, Jensen J, McMullin RT, Wang L, Leavitt SD (2022) Providing scale to a known taxonomic unknown—At least a 70-fold increase in species diversity in a cosmopolitan nominal taxon of lichen-forming fungi. Journal of Fungi (Basel, Switzerland) 8: 490. https://doi.org/10.3390/jof8050490