Published October 25, 2025 | Version v1
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Rapid diagnostic methods for Tuberculosis

Description

Tuberculosis (TB) is still a serious global health concern that requires prompt and precise diagnosis in order to be effectively controlled. Conventional techniques like culture and smear microscopy have drawbacks such slow turnaround times and poor sensitivity. The speed and precision of detection have been greatly increased by modern quick diagnostics, including as automated culture systems, antigen detection tests, and nucleic acid amplification tests (Xpert MTB/RIF, line probe assays). Drug resistance discovery is significantly improved by new technologies like next-generation sequencing and loop-mediated isothermal amplification. Notwithstanding their benefits, problems with infrastructure, expense, and extrapulmonary TB's low sensitivity still exist. It is crucial to incorporate these quick techniques into health systems in order to facilitate early detection, timely treatment, and a decrease in the spread of tuberculosis globally.

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Dates

Accepted
2025-10-25
Accepted

References

  • 1. Dinnes J, Deeks JJ, Kunst H, Gibson A, Cummins E, Waugh N, Lalvani A. A systematic review of rapid diagnostic tests for the detection of tuberculosis infection. Health Technol Assess. 2007;11(3):1-196. PMID: 17266837. NCBI +1
  • 2. World Health Organization. Manual for selection of molecular WHO-recommended rapid diagnostic tests for detection of tuberculosis and drug-resistant tuberculosis. Geneva: WHO; 2022. ISBN: 978-92-4-004257-5. World Health Organization +1
  • 3. World Health Organization. WHO consolidated guidelines on tuberculosis. Module 3: Diagnosis – Rapid diagnostics for tuberculosis detection. 3rd ed. Geneva: WHO; 2024. ISBN: 978-92-4-008950-1. World Health Organization +1
  • 4. Chang D, He Z, Li Y, Xu X, Wang C, Zeng J. Improved conventional and new approaches in the diagnosis of tuberculosis. Front Microbiol. 2022;13:924410. doi:10.3389/fmicb.2022.924410. Frontiers
  • 5. Patel V, et al. Impact of molecular diagnostic tests on diagnostic and treatment delays in tuberculosis: a systematic review and meta-analysis. BMC Infect Dis. 2022;22:… doi:10.1186/s12879-022-07855-9. BioMed Central
  • 6. ATS/IDSA/CDC. Clinical Practice Guidelines: Diagnosis of Tuberculosis in Adults and Children. Clin Infect Dis. 2021;… Infectious Diseases Society of America
  • 7. Engel N, Ochodo E A, Karanja P W, Schmidt B M, Janssen R, Steingart K R, et al. Rapid molecular tests for tuberculosis and tuberculosis drug resistance: a qualitative evidence synthesis of recipient and provider views. Cochrane Database Syst Rev. 2022;4:CD014877. PubMed +2 Cochrane +2
  • 8. World Health Organization. Rapid communication: molecular assays as initial tests for the diagnosis of tuberculosis and rifampicin resistance. Geneva: WHO Global Tuberculosis Programme; 2020. ISBN: 978-92-4-000033-9. World Health Organization
  • 9. Nandlal L, et al. Rapid molecular assays for the diagnosis of drug-resistant tuberculosis. Infect Drug Resist. 2022;15:6009-6023. PubMed
  • 10. Weyer K, Mirzayev F, Migliori G B, Van Gemert W, D'Ambrosio L, Zignol M, et al. Rapid molecular TB diagnosis: evidence, policy making and global implementation of Xpert MTB/RIF. Int J Tuberc Lung Dis. 2014;18(7):780-792. PubMed +1
  • 11. A Comprehensive Review of the Diagnostics for Pediatric Tuberculosis Based on Assay Time, Ease of Operation, and Performance. Microorganisms. 2025;13(1):178. DOI:10.3390/microorganisms13010178. MDPI
  • 12. Huang Z, Zhang G, Lyon C J, Hu T Y, Lu S. Outlook for CRISPR-based tuberculosis assays now in their infancy. Front Immunol. 2023;14:1172035. DOI:10.3389/fimmu.2023.1172035. Frontiers
  • 13. Nair S A. Revolutionary Advances in Diagnostic Tests for Tuberculosis Infection and Disease. Biomed J Sci & Tech Res. 2023;50(5). BJSTR.MS.ID.008002. Biomedres