Published October 21, 2025 | Version v1

A guide to selecting high-performing antibodies for ASM (UniProt ID: P17405) for use in western blot and immunoprecipitation

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This report provides a guide to selecting high-quality commercial antibodies against ASM for use in western blot and immunoprecipitation, utilizing a knockdown cell line as a negative control. This work was supported by a grant from the CQDM (by a grant from the Ministère de l’Économie, de l’Innovation et de l’Énergie du Québec). This research was also partly funded by the G-Can (GBA1-Canada) initiative, an open-science collaboration dedicated to advancing research on Parkinson's disease associated with GBA1 mutations. G-Can is supported by The Hilary & Galen Weston Foundation, Silverstein Foundation, and J. Sebastian van Berkom and Ghislaine Saucier.

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SMPD1_ASM_YCharOS report.pdf

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