Published October 14, 2025
| Version v1
Journal article
Open
Multilocus phylogeny and morphology reveal two new species of Lepiota (Agaricales, Verrucosporaceae) from southwestern China
Authors/Creators
- 1. Chinese Academy of Forestry, Guangzhou, China
- 2. College of Life Science, Xinxiang, China
Description
Based on phylogenetic and morphological evidence, two new Lepiota sect. Lepiota species collected from southwestern China are described and illustrated, namely L. brunneophora and L. ochraceosquamea. Lepiota brunneophora has a pileus with yellowish-brown to brown squamules and striations, a reddish-brown to purplish-brown stipe covered with whitish, floccose squamules, and penguin-shaped basidiospores with a narrowed apex. Lepiota ochraceosquamea is characterized by dark yellowish-brown to light ochre squamules on the pileus, a whitish annulus, broadly fusiform basidiospores, and a trichodermal pileus covering composed of subcylindrical terminal elements, narrowing toward the apex and lacking basal short elements. A comprehensive phylogenetic framework within L. sect. Lepiota, including agaricoid and sequestrate species, is also provided.
Files
MC_article_163999.pdf
Files
(9.9 MB)
| Name | Size | Download all |
|---|---|---|
|
md5:d7939bbaabfc2f61dde75e99a89f1064
|
9.9 MB | Preview Download |
System files
(145.6 kB)
| Name | Size | Download all |
|---|---|---|
|
md5:f055f5b197c61df801727105fd850d39
|
145.6 kB | Download |
Linked records
Additional details
References
- Ahamed M, Verma K, Dutta AK, Sharma YP (2023) Lepiota albofloccosa, a new species in sect. Lepiota (Agaricaceae, Agaricales) from Northwestern Himalayas of Jammu and Kashmir, India. Phytotaxa 607(1): 72–84. https://doi.org/10.11646/phytotaxa.607.1.6
- Bashir H, Usman M, Khalid AN (2020) Lepiota cholistanensis a new species of Lepiota (Agaricaceae: Basidiomycota) from Cholistan desert, Pakistan. Phytotaxa 455(4): 267–276. https://doi.org/10.11646/phytotaxa.455.4.4
- Bau T, Li HJ, Bao HY, Li Y (2024) A revised checklist of poisonous mushrooms in China. Journal of Fungal Research 22(4): 301–321. https://doi.org/10.13341/j.jfr.2024.0010
- Bon M (1981) Clé monographique des 'lépiotes' d'Europe. Documents Mycologiques 11(43): 1–77.
- Bon M (1991) Les genres Echinoderma (Locq. ex Bon) st. nov. et Rugosomyces Raithelhuber ss. lato. Documents Mycologiques 21(82): 61–66.
- Bon M (1993) Novitates 4. Famille Lepiotaceae Roze ex Overeem. Documents Mycologiques 22(88): 27–32.
- Candusso M, Lanzoni G (1990) Lepiota s.l. Fungi Europaei 4. Libreria editrice Giovanna Biella, Saronno, 322 pp.
- Castellano MA (1995) NATS truffle and truffle-like fungi. 4: Amogaster viridigleba gen. et sp. nov., a new truffle-like fungus from the Sierra Nevada. Mycotaxon 55: 185–188.
- Dai YC, Zhou LW, Yang ZL, Wen HA, Bau T, Li TH (2010) A revised checklist of edible fungi in China. Mycosystema 29(1): 1–21.
- Gardes M, Bruns TD (1993) ITS primers with enhanced specificity for basidiomycetes—Application to the identification of mycorrhizae and rusts. Molecular Ecology 2: 113–118. https://doi.org/10.1111/j.1365-294X.1993.tb00005.x
- Ge ZW, Smith ME (2013) Phylogenetic analysis of rDNA sequences indicates that the sequestrate Amogaster viridiglebus is derived from within the agaricoid genus Lepiota (Agaricaceae). Mycological Progress 12: 151–155. https://doi.org/10.1007/s11557-012-0841-y
- Guindon S, Dufayard JF, Lefort V, Anisimova M, Hordijk W, Gascuel O (2010) New algorithms and methods to estimate maximum-likelihood phylogenies: Assessing the performance of PhyML 3.0. Systematic Biology 59: 307–321. https://doi.org/10.1093/sysbio/syq010
- Guzmán G, Guzmán-Davalos L (1992) A checklist of the lepiotaceous fungi. Koeltz Scientific Books, Champaign, 216 pp.
- Haqnawaz M, Niazi AR, Usman M, Khalid AN (2022) Lepiota sindhudeltana sp. nov. (Agaricaceae; Basidiomycota) from Punjab, Pakistan. Phytotaxa 550(3): 253–262. https://doi.org/10.11646/phytotaxa.550.3.5
- Hausknecht A, Pidlich-Aigener H (2005) Lepiotaceae (Schirmlinge) in Österreich 2. Die Gattung Lepiota. Österreichische Zeitschrift für Pilzkunde 14: 41–78.
- Hou YJ, Ge ZW (2020) New species of Echinoderma and Lepiota (Agaricaceae) from China. Phytotaxa 447(4): 221–236. https://doi.org/10.11646/phytotaxa.447.4.1
- Jülich W (1982) Higher taxa of Basidiomycetes. Bibliotheca Mycologica 85: 393.
- Kalichman J, Kirk PM, Matheny PB (2020) A compendium of generic names of agarics and Agaricales. Taxon 69(3): 425–447. https://doi.org/10.1002/tax.12240
- Kalyaanamoorthy S, Minh BQ, Wong T, von Haeseler A, Jermiin LS (2017) ModelFinder: Fast model selection for accurate phylogenetic estimates. Nature Methods 14(6): 587–589. https://doi.org/10.1038/nmeth.4285
- Katoh K, Standley DM (2013) MAFFT: multiple sequence alignment software version 7: improvements in performance and usability. Molecular Biology and Evolution 30(4): 772–780. https://doi.org/10.1093/molbev/mst010
- Kaygusuz O (2022) Lepiota cremea, a novel species of Lepiota sect. Lepiota (Agaricaceae s.l., Agaricales) from Turkey. Phytotaxa 555(4): 291–300. https://doi.org/10.11646/phytotaxa.555.4.2
- Kirk PM, Cannon PF, Minter DW, Stalpers JA (2008) Ainsworth & Bisby's dictionary of the fungi, 10th edn. CABI, Wallingford, 771 pp. https://doi.org/10.1079/9780851998268.0000
- Knudsen H (1980) A revision of Lepiota sect. Echinatae and Amyloideae (Agaricaceae) in Europe. Botanisk Tidsskrift 75: 121–155.
- Kornerup A, Wanscher JH (1981) Taschenlexikon der Farben. 3. Muster-Schmidt, Göttingen, 78 pp.
- Lange JE (1915) Studies in the Agarics of Denmark. Part II. Amanita. Lepiota. Coprinus. Dansk Botanisk Arkiv 2(3): 1–60.
- Lange JE (1935) Flora Agaricina Danica, I. Recato, Copenhagen, 1–40.
- Lebel T, Vellinga EC (2013) Description and affinities of a sequestrate Lepiota (Agaricaceae) from Australia. Mycological Progress 12: 525–532. https://doi.org/10.1007/s11557-012-0859-1
- Li JL, Wang S, Yi J, Wang L, Zhou SL (2013) A modified CTAB protocol for plant DNA extraction. Zhiwu Xuebao 48(1): 72–78. https://doi.org/10.3724/SP.J.1259.2013.00072
- Liang JF (2007) Study on taxonomy of Lepiota from China and molecular phylogeny of the genus with notes on population genetics of L. cristata. PhD Thesis, Kunming Institute of Botany, the Chinese Academy of Sciences, China.
- Liang JF (2012) Lepiota amplicystidiata, a new species from Tibet. Sydowia 64(2): 245–254.
- Liang JF (2016) Taxonomy and phylogeny in Lepiota sect. Stenosporae from China. Mycologia 108(1): 56–69. https://doi.org/10.3852/14-192
- Liang JF, Yang ZL (2011) A new species of Lepiota (Agaricaceae) from southwestern China. Mycotaxon 117: 359–363. https://doi.org/10.5248/117.359
- Liang JF, Yang ZL (2012) Lepiota nigrosquamosa, a new species from China. Nova Hedwigia 96(1–2): 213–220. https://doi.org/10.1127/0029-5035/2012/0054
- Liang JF, Xu J, Yang ZL (2009) Divergence, dispersal and recombination in Lepiota cristata from China. Fungal Diversity 38: 105–124.
- Liang JF, Yang ZL, Xu DP (2011) A new species of Lepiota from China. Mycologia 103(4): 820–830. https://doi.org/10.3852/10-216
- Liang JF, Yu F, Lu JK, Wang SK, Song J (2018) Morphological and molecular evidence for two new species in Lepiota from China. Mycologia 110(3): 494–501. https://doi.org/10.1080/00275514.2018.1464333
- Minh BQ, Nguyen MA, von Haeseler A (2013) Ultrafast approximation for phylogenetic bootstrap. Molecular Biology and Evolution 30(5): 1188–1195. https://doi.org/10.1093/molbev/mst024
- Murrill WA (1951) New agarics from Florid. Mycologia 43(2): 235–239. https://doi.org/10.1080/00275514.1951.12024123
- Nguyen LT, Schmidt HA, Haeseler V, Minh BQ (2015) IQ-TREE: A fast and effective stochastic algorithm for estimating Maximum-Likelihood phylogenies. Molecular Biology and Evolution 32(1): 268–274. https://doi.org/10.1093/molbev/msu300
- Niazi AR, Asif M, Izhar A, Khalid AN (2021) A new species of Lepiota (Agaricaceae) from Punjab, Pakistan. Phytotaxa 511(2): 163–174. https://doi.org/10.11646/phytotaxa.511.2.4
- Paz A, Lavoise C (2024) Balsamia sotxoi y Lepiota faiae-bravae, dos nuevas especies de hongos secotioides. Boletín Micológico de FAMCAL 19: 9–25.
- Pegler DN (1986) Agaric Flora of Sri Lanka. Kew Bulletin Additional Series 12: 285–313.
- Qu H, Cai Q, Redhead SA, Chen X, Ge ZW, Yang ZL (2025) Exploring criteria for constructing suprageneric classifications of fungi in the genomic era: A case study of suborders Agaricineae, Pluteineae, and Tricholomatineae (Agaricales). Fungal Diversity. https://doi.org/10.1007/s13225-025-00557-y
- Rea C (1922) British Basidiomycetae: a handbook to the larger British Fungi. Cambridge, Univesity press, 799 pp. https://doi.org/10.5962/bhl.title.46431
- Rehman A, Usman M, Afshan NUS, Khalid AN (2024) Taxonomy and phylogeny reveal two novel species of genus Lepiota (Agaricaceae, Agaricales) from Punjab, Pakistan. Plant Systematics and Evolution 310: 8. [1–12] https://doi.org/10.1007/s00606-024-01891-5
- Ronquist F, Teslenko M, Mark VDP, Ayres DL, Darling A, Höhna S, Larget B, Liu L, Suchard MA, Huelsenbeck JP (2012) MrBayes 3.2: Efficient Bayesian phylogenetic inference and model choice across a large model space. Systematic Biology 61(3): 539–542. https://doi.org/10.1093/sysbio/sys029
- Saccardo PA (1887) Sylloge fungorum omnium hucusque cognitorum 5. Sumptibus auctoris, Patavii, 1146 pp.
- Salichanh T, Sysouphanthong P, Thongklang N, Luangharn T (2024) Cystolepiota flavolamellata (Verrucosporaceae, Agaricales), a new species from Southeast Asia. Phytotaxa 645(1): 18–28. https://doi.org/10.11646/phytotaxa.645.1.2
- Sarawi S, Shi YN, Lotz-Winter H, Reschke K, Bode HB, Piepenbring M (2022) Occurrence and chemotaxonomical analysis of amatoxins in Lepiota spp. (Agaricales). Phytochemistry 195: 113069. https://doi.org/10.1016/j.phytochem.2021.113069
- Sarawi S, Piepenbring M, Reschke K (2025) Phylogenetic and taxonomic reassessment of the genera Echinoderma and Lepiota. Fungal Systematics and Evolution 15: 235–263. https://doi.org/10.3114/fuse.2025.15.11
- Sgambelluri RM, Epis S, Sassera D, Luo H, Angelos ER, Walton JD (2014) Profiling of amatoxins and phallotoxins in the genus Lepiota by liquid chromatography combined with UV absorbance and mass spectrometry. Toxins 6(8): 2336–2347. https://doi.org/10.3390/toxins6082336
- Singer R (1986) The Agaricales in modern taxonomy. 4th edn. Koeltz Scientific Books, Koenigstein, 903 pp.
- Sysouphanthong P, Hyde KD, Chukeatirpte E, Vellinga EC (2011) A review of genus Lepiota and its distribution in east Asia. Current Research in Environmental & Applied Mycology 1(2): 161–176.
- Sysouphanthong P, Hyde KD, Chukeatirote E, Bahkali AH, Vellinga EC (2012) Lepiota (Agaricales) in northern Thailand-2 Lepiota section Lepiota. Cryptogamie. Mycologie 33(1): 25–42. https://doi.org/10.7872/crym.v33.iss1.2012.025
- Thiers B (2025) [continuously updated] Index Herbariorum: A global directory of public herbaria and associated staff. http://sweetgum.nybg.org/ih [Accessed 31 May 2025]
- Vellinga EC (1988) Glossary. In: Bas C, Kuyper TW, Noordeloos ME, Vellinga EC (Eds) Flora Agaricina Neerlandica. Critical monographs on families of agarics and boleti occurring in the Netherlands vol. 1. A.A. Balkema Publishers, Lisse, 54–64.
- Vellinga EC (2001) Lepiota (Pers.: Fr.) S.F. Gray. In: Noordeloos ME, Kuyper TW, Vellinga EC (Eds) Flora Agaricina Neerlandica. Critical monographs on families of agarics and boleti occurring in the Netherlands vol. 5. A.A. Balkema Publishers, Lisse, 109–151.
- Vellinga EC (2003) Phylogeny of Lepiota (Agaricaceae) – Evidence from nrITS and nrLSU sequences. Mycological Progress 2(4): 305–322. https://doi.org/10.1007/s11557-006-0068-x
- Vellinga EC (2004a) Genera in the family Agaricaceae: Evidence from nrITS and nrLSU sequences. Mycological Progress 108(4): 354–377. https://doi.org/10.1017/s0953756204009700
- Vellinga EC (2004b) Ecology and distribution of lepiotaceous fungi (Agaricaceae) - A review. Nova Hedwigia 78(3–4): 273–299. https://doi.org/10.1127/0029-5035/2004/0078-0273
- Vidal JM, Juste P, García F, Bellanger JM, Moreau PA (2015) Hongos secotioides del género Lepiota: Dos nuevas especies, dos nuevas combinaciones y reevaluación del género Cribrospora. Boletín Micológico de FAMCAL 11: 47–71.
- Vilgalys R, Hester M (1990) Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. Journal of Bacteriology 172(8): 4238–4246. https://doi.org/10.1128/jb.172.8.4238-4246.1990
- Wasser SP (1978) New taxonomic combinations and new taxons in the family Agaricaceae Fr. Ukrainian Botanical Journal 35(5): 516–519.
- White TJ, Bruns T, Lee S, Taylor JW (1990) Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In: Innis MA, Gelfand DH, Sninsky JJ, White TJ (Eds) PCR protocols: a guide to the methods and applications. Academic Press, New York, 315–322. https://doi.org/10.1016/B978-0-12-372180-8.50042-1
- Wieland T, Faulstich H (1991) Fifty years of amanitin. Experientia 47(11–12): 1186–1193. https://doi.org/10.1007/BF01918382
- Xiang CY, Gao FL, Jakovlić I, Lei HP, Hu Y, Zhang H, Zou H, Wang GT, Zhang D (2023) Using phyloSuite for molecular phylogeny and tree-based analyses. iMeta 2(1): e87. https://doi.org/10.1002/imt2.87
- Yang ZL, Ge ZW, Liang JF (2019) Fungi lepiotoidei (Agaricaceae). In: Consilio florarum cryptogamarum sinicarum academiae sinicae (Eds) Flora fungorum sinicorum vol. 52. Science Press, Beijing, 228 pp.
- Yang KL, Lin JY, Li GM, Li TH, Yang ZL (2024) Rediscovering Leucoagaricus sinicus, with the recognition of Leucoagaricus and Leucocoprinus as separate genera, and two new genera in Agaricaceae (Basidiomycota). Phytotaxa 676(3): 199–255. https://doi.org/10.11646/phytotaxa.676.3.1
- Zhang D, Gao FL, Jakovlić I, Zou H, Zhang J, Li WX, Wang GT (2020) PhyloSuite: An integrated and scalable desktop platform for streamlined molecular sequence data management and evolutionary phylogenetics studies. Molecular Ecology Resources 20(1): 348–355. https://doi.org/10.1111/1755-0998.13096
- Zhou LL (2010) Taxonomy and molecular phylogeny of Lepiota and Leucoagaricus from China. Master thesis, Jiangxi Agricultural University.