Development and Validation of RP-HPLC Method for the estimation of vitexin in Convolvulus arvensis and Neurada procumbens

Vitexin, a C-glycosyl flavonoid was estimated in the extracts of Convolvulus arvensis and Neurada procumbens by reversed phase high-performance liquid chromatography (RP-HPLC). C18 column was used as stationary phase and methanol-0.05 % o-phosphoric acid in the ratio of 20:80 was used to separate the compounds from the extracts by isocratic program. Vitexin was detected at 340 nm by a UV-Visible detector, which showed a sharp peak with the retention time of 6.692 minutes. The quantitative study revealed that 0.727 mg/g of vitexin in the extract of Convolvulus arvensis and 0.0372 mg/g of vitexin in the extract of Neurada procumbens. The linearity range was found to between 100 ng to 200 µg/ml with the correlation coefficient of r2≥0.9994. The limit of detection and limit of quantification of vitexin were found to be 30 and 100 ng/ml. The developed method is simple, precise and accurate for the quantification of vitexin in the extracts and might be helpful in the quality control of these plants.