Verification of analytical performance of Complement C3 on the Abbott Alinity ci®: Experience of the central laboratory of Mohammed VI University Hospital of Oujda

The verification of analytical methods is a requirement outlined by the International Organization for Standardization (ISO). This process involves evaluating the performance of an analytical method according to a well-defined protocol and subsequently comparing it with pre-established analytical objectives. 

In our study, we conducted an evaluation of the analytical performance of Complement C3 using the Abbott kit on the Alinity CI analyzer in the biochemistry laboratory of Mohammed VI University Hospital in Oujda. The methodology employed adheres to the recommendations of  the  French accreditation  committee  (COFRAC) accreditation  technical  guide SH  GTA  04, focusing on  the  assessment of  reproducibility  and repeatability.The results obtained demonstrate good repeatability, with CV1 = 1.04%, CV2 = 1.13%, and CV3 = 0.97% for levels 1, 2, and 3, respectively. Intra-laboratory reproducibility was also satisfactory for levels 1, 2, and 3, with CV1 = 4.8%, CV2 = 3.13%, and CV3 = 2.9%. Confirming the performance of this method is essential, given the qualitative and quantitative importance of Complement C3 measurement in our daily practice. It's important to note that the accuracy and reliability of examination results are influenced not only by laboratory personnel, equipment, and environmental conditions, but also by the methods utilized and their eventual validation or verification.