Additional data from Milivojev et al., "Light-modulated stem cells in the camera-type eye of an annelid model for adult brain plasticity"

The files deposited here represent the data related to the article "Light-modulated stem cells in the camera-type eye of an annelid model for adult brain plasticity" by Milivojev et al.

Files included in this repository are as follows:

• Additional data displaying gating information of fluorescence-activated cell sorting (FACS) and genome-mapping of single cell RNA-sequencing (scRNA-seq) libraries.
  • FACS_plots_1: FACS gating of single cell suspensions obtained from head tissue of (A) immature, (B) premature, (C) female and (D) male worms.
  • FACS_plots_2: FACS gating of single cell suspensions obtained from head tissue of (A) EdU-labeled and (B) unlabeled control worms sampled at the same time.
  • FACS_plots_3: (A-C) FACS gating of single cell suspensions obtained from head tissue of c-ops1Δ8/Δ8 worms; the genotype is presented as three replicated with the suffixes _1, _2 and _3.
  • FACS_plots_4: (A-C) FACS gating of single cell suspensions obtained from head tissue of WT worms corresponsing to c-ops1Δ8/Δ8 mutants; the genotype is presented as three replicated with the suffixes _1, _2 and _3.
  • Genome_mapping_1: Overview of results and statistics of genome-mapping of raw sequencing data of (A) immature, (B) premature and (C) female library.
  • Genome_mapping_2: Overview of results and statistics of genome-mapping of raw sequencing data of (A) male, (B) EdU-labeled and (C) corresponding unlabeled sampling time-matched control library.
  • Genome_mapping_3: (A-C) Overview of results and statistics of genome-mapping of raw sequencing data of c-ops1Δ8/Δ8 worms; the genotype is presented as three replicated with the suffixes _1, _2 and _3.
  • Genome_mapping_4: (A-C) Overview of results and statistics of genome-mapping of raw sequencing data of WT worms corresponding to c-ops1Δ8/Δ8 mutants; the genotype is presented as three replicated with the suffixes _1, _2 and _3.
• All R code used for processing, analysis and visualization of the data generated in the scope of the study (in the form of an R notebook), as well as all relevant R objects generated as output in this manner (in the form of an RData file). The objects in the RData file are named consistently with their variables in the code.