Multiple-target tracing (MTT) algorithm probes molecular dynamics at cell surface

Single fluorescence molecule microscopy provides a privileged approach achieving a spatial resolution close to the molecular scale (1-5). The position of an isolated fluorescent molecule is accurately determined by the center of the signal. The counterpart is a necessity for imaging sparse fluorescent molecules. Consequently, single particle tracking (SPT) is only amenable when handling a very limited number of targets at the same time.