Published October 13, 2025 | Version v1
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Qualitative phytochemical analysis and antioxidant activity of Leersia hexandra Sw. (Poaceae)

  • 1. Department of Botany, Department of Botany, Tripura University, Suryamaninagar, Tripura, India
  • 2. Department of Biotechnology, Orissa University of Technology and Research, Bhubaneswar, Odisha, India
  • 3. Ambika Prasad Research Foundation, Odisha, India
  • 4. Amaltas Institute of Ayurveda, Amaltas Ayurvedic Hospital and Research Centre, Dewas, Madhya Pradesh, India

Description

Leersia hexandra is a perennial aquatic grass and known for its ecological adaptability and medicinal properties. Present study aimed to evaluate the phytochemicals and antioxidant activity of L. hexandra. Phytochemical analysis revealed that solvent polarity strongly influenced metabolite extraction. Non-polar extracts did not show detectable compounds, whereas ethanol and aqueous extracts contained tannins, flavonoids, and reducing sugars. The aqueous extract exhibited the highest richness of bioactive compounds and antioxidant activity. The ethyl acetate: methanol: water system showed strong radical scavenging activity with an Rf of 0.983, followed by moderate activity in chloroform: ethyl acetate: formic acid and minimal response in non-polar solvents. The results highlighted the predominance of polar bioactive compounds in L. hexandra, supporting its potential as a natural antioxidant source and a candidate for future pharmacological and ecological applications as fodder grass.

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