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Published November 20, 2025 | Version v2
Preprint Open

Preprint for: Micropatterning and Nanodropletting of Titanium by Shifted Surface Laser Texturing Significantly Enhances In Vitro Osteogenesis of Healthy and Osteoporotic Mesenchymal Stromal Cells

Authors/Creators

  • 1. Experimental Trauma Surgery, Department of Trauma Surgery, University Regensburg Medical Centre, Regensburg, Germany
  • 2. Department of Musculoskeletal Tissue Regeneration and Department of Orthopedics, Orthopedic Hos-pital König-Ludwig-Haus, Julius-Maximilians-University Würzburg, Germany
  • 3. Department of Spinal Surgery, The Second Hospital of Dalian Medical University, Dalian Medical University, Dalian, China
  • 4. New Technologies Research Centre, University of West Bohemia, Pilsen, Czech Republic
  • 5. Department of Machining Technology, Faculty of Mechanical Engineering, University of West Bohemia, Czech Republic
  • 6. Department of Orthopedics and Trauma Surgery, Musculoskeletal University Centre Munich (MUM), Ludwig-Maximilians-University (LMU) Hospital, Munich, Germany
  • 7. Department of Conservative Dentistry and Periodontology, University Hospital Regensburg, Regens-burg, Germany
  • 8. Department of Operative Dentistry and Periodontology, Center for Dental Medicine, Medical Cen-ter—University of Freiburg, Faculty of Medicine, University of Freiburg, Germany
  • 9. Západočeská Univerzita v Plzni
  • 10. ROR icon University of West Bohemia

Description

In this experimental paper, titanium (Ti) scaffolds were subjected to shifted laser surface texturing (sLST) using a nanosecond pulsed laser to create an open pore macrotopography with micro-and nano- Ti droplets. In contrast to conventional laser texturing, which leads to high heat accumulation; in sLST, the frequency of laser pulses is low, allowing for resolidification, thereby creating a surface with abundant coverage micro-/nanodroplets. The main objective was to compare the cellular responses of human mesenchymal stromal cells (hMSCs) on sLST-textured Ti surfaces (LT-Ti) for the first time with standard sand-blasted, acid-etched surfaces (SLA-Ti). In-depth analyses of cell survival, proliferation, shape, mineralization, and gene expression were performed.

Files

Preprint_Stich et al. 2025_main manuscript_submitted to MDPI_watermark.pdf

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Additional details

Identifiers

DOI
10.5281/zenodo.17338495

Related works

Is cited by
Journal article: 10.3390/jfb16110401 (DOI)
Is supplemented by
Dataset: 10.5281/zenodo.17338495 (DOI)

Funding

European Union
Operational Programme Johanes Amos Comenius, call Excellent Research, co funded by the European Union, administered by the Ministry of Education, Sports and Youth CZ.02.01.01/00/22_008/0004634
European Union
Project Nr. 349. OSTEOMET: Augmented treatment of osteoporotic bone with innovative organic-inorganic nanoparticle functionalized metal surfaces 349
European Union
Project number: BYCZ01-129: KT-Dent: Cross-border transfer of knowledge in the field of advanced dental materials 129