Thermal shifts can override the effects of transcription-targeting antibiotics

------------------------------------------------------------
DATA & FILE OVERVIEW
------------------------------------------------------------

1. File List:
(Folders contained in the dataset, with brief descriptions)

01_Flow_Cytometry:
This folder contains all flow cytometry data used in the project. 
The folder structure is organized according to the figures in the paper.

    • 01_GyrA_GyrB_RpoB – Related to Section "2.6 Global transcription regulator levels increase during dual stresses".
    • 02_Resistance_regulators – Related to Section "Thermal stresses alone induce antibiotic resistance regulators and efflux pump expression".

Note:
All flow cytometry data were acquired using an ACEA NovoCyte Flow Cytometer 
(ACEA Biosciences Inc., San Diego, USA). 
All raw flow cytometry data are provided as *.csv files, where each row 
represents the fluorescence value (FITC-H channel) corresponding to a single cell.

02_Microscopy:
This folder contains Nikon .nd2 microscopy image files. 
Each condition includes 20 different panels of raw microscopy images.

03_Spectrophotometry:
    • 01_ATP – Related to Section "2.3 Temperature-dominant genes are associated with metabolic regulation".

04_Codes:
All custom-made codes used for data analysis in this study.

05_Supplementary_data:
    • Feature_of_genes.xlsx – List of genes with different features considered for the random forest ML analysis.
    • Shifter_genes.xlsx – List of temperature-dominant genes identified in the study.

2. Are there multiple versions of the dataset? 
No.

------------------------------------------------------------
METHODOLOGICAL INFORMATION
------------------------------------------------------------

1. Description of methods used for data collection/generation:
Collection and data generation are described in the "Materials and Methods" section of the main manuscript.

2. Methods for data processing:
Data processing methods and software packages used are described in the "Materials and Methods" section of the main manuscript.