Comprehensive Qualitative and Quantitative Analysis of Flavonoids in Dandelion (Taraxacum officinale) Flowers and Food Products

Files draft_Proof_2024_JAFC_Taraxacum.pdf and TOF_JAFC_SM.pdf are related to published article.

Abstract

Taraxacum officinale, a cosmopolitan perennial, is widely used as an edible, fodder, honey, and medicinal plant. Interestingly, detailed LC-QTOF-MS profiling of the flower extract revealed the presence of several flavonoid signals, most likely lacking literature confirmation. Targeted isolation, including extraction based on DoE planning, led to the isolation of 11 flavonoids, three of which were new (16, 18, and 33). Their structures were determined by the NMR technique. Both biflavones (16 and 18) were structured by two luteolin molecules, linked by a C–C bond through IB (C-6′) and IIA (C-6″ or C-8″) rings. Novel flavonolignan (33) was composed of tricin and an unusual carboxyl function containing a lignan moiety. The content of 28 flavonoids (glycosides, aglycones, biflavones, and flavonolignans) was determined by the LC–MS/MS method in raw flowers and dandelion syrups and tincture. Multivariate analyses showed the separation of spring and autumn flowers and a high diversity of food products and helped to identify metabolites correlated with the samples.

“This document is the Accepted Manuscript version of a Published Work that appeared in final form in Journal of Agricultural and Food Chemistry, copyright © 2024 American Chemical Society after peer review and technical editing by the publisher. To access the final edited and published work see https://pubs.acs.org/doi/abs/10.1021/acs.jafc.4c03108.”

The other files contains data sets related to above article:

TOF_extracts_matrix1.csv - data matrix with flavonoid content in dandelion flower extracts, used for multivariate analyses using the Metaboanalyst platform

TOF_Flavonoid_Calibration_Curves_1.xlsx - flavonoid standard calibration curves

TOF_syrups_matrix1.csv - data matrix with flavonoid content in dandelion flower syrups and tincture, used for multivariate analyses using the Metaboanalyst platform

94_TOF_Flavonoid_CE50_P_RA2_1_4157.d.zip - Raw data from Bruker UHPLC-HR-QTOF-MS positive ionization chromatographic analysis of dandelion flower flavonoid fraction

98_TOF_Flavonoid_CE50_N_RA2_1_4161.d.zip - Raw data from Bruker UHPLC-HR-QTOF-MS negative ionization chromatographic analysis of dandelion flower flavonoid fraction

This work is funded in part by the National Science Centre – Preludium project ID: 2022/45/N/NZ9/03440.