Published October 1, 2025 | Version v1
Journal article Open

New species of Phanerotomella (Hymenoptera, Braconidae, Cheloninae) based on morphological and molecular evidence

  • 1. Ministry of Agriculture and Rural Affairs, Key Lab of Molecular Biology of Crop Pathogens and Insects, Zhejiang University, Hangzhou, China|State Key Lab of Rice Biology and Breeding, Zhejiang University, Hangzhou, China|Zhejiang Provincial Key Laboratory of Biology and Ecological Regulation of Crop Pathogens and Insects, Zhejiang University, Hangzhou, China|Institute of Insect Sciences, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, China
  • 2. Zhejiang Provincial Key Laboratory of Biology and Ecological Regulation of Crop Pathogens and Insects, Zhejiang University, Hangzhou, China|Ministry of Agriculture and Rural Affairs, Key Lab of Molecular Biology of Crop Pathogens and Insects, Zhejiang University, Hangzhou, China|Institute of Insect Sciences, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, China|State Key Lab of Rice Biology and Breeding, Zhejiang University, Hangzhou, China
  • 3. Institute of Insect Sciences, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, China
  • 4. Ministry of Agriculture and Rural Affairs, Key Lab of Molecular Biology of Crop Pathogens and Insects, Zhejiang University, Hangzhou, China|Institute of Insect Sciences, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, China|State Key Lab of Rice Biology and Breeding, Zhejiang University, Hangzhou, China|Zhejiang Provincial Key Laboratory of Biology and Ecological Regulation of Crop Pathogens and Insects, Zhejiang University, Hangzhou, China
  • 5. State Key Lab of Rice Biology and Breeding, Zhejiang University, Hangzhou, China|Institute of Insect Sciences, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou, China|Zhejiang Provincial Key Laboratory of Biology and Ecological Regulation of Crop Pathogens and Insects, Zhejiang University, Hangzhou, China|Ministry of Agriculture and Rural Affairs, Key Lab of Molecular Biology of Crop Pathogens and Insects, Zhejiang University, Hangzhou, China

Description

The genus Phanerotomella Szépligeti, 1900 (Hymenoptera, Braconidae, Cheloninae, Phanerotomini), is restricted to the Old World, with no records from the Nearctic or Neotropical Regions. Reliably species-identified COI sequences are scarce for this genus in public databases, hindering comprehensive phylogenetic and DNA-barcoding analyses.

This study provides 29 COI sequences for this genus. Two species delimitation approaches, combined with morphological evidence, were employed to delimit species. The findings indicated the existence of 16 species, including one new species: P. nitidifascia Fang, He, van Achterberg, sp. nov. and one newly-recorded species: P. namkyensis Sigwalt, 1978. Additionally, we provide the first available COI sequence for the Chinese endemic genus Siniphanerotomella.

Files

BDJ_article_171754.pdf

Files (1.7 MB)

Name Size Download all
md5:b4a3e2310bcf0a9165a5adf157f1574e
1.7 MB Preview Download

System files (103.9 kB)

Name Size Download all
md5:b203027887d58f1f7e66edb77bf4b97a
103.9 kB Download

Linked records

Additional details

References

  • Chen JH, Ji QE (2003) Systematic studies on Cheloninae of China (Hymenoptera: Braconidae). Fujian Scientific Publishers, Fuchow, 328 pp.
  • Cruaud A, Nidelet S, Arnal P, Weber A, Fusu L, Gumovsky A, Huber J, Polaszek A, Rasplus J (2019) Optimized DNA extraction and library preparation for minute arthropods: Application to target enrichment in chalcid wasps used for biocontrol. Molecular Ecology Resources 19 (3): 702‑710. https://doi.org/10.1111/1755-0998.13006
  • Dalla TGG (1898) Catalogus Hymenopterorum. Volume IV. Braconidae. Guilelmi Engelmann, Lipsiae, 323 pp.
  • Fahringer J (1934) Opuscula braconologica. Band 3. Palaearktischen Region. Lieferung 5–8: 321–594. Fritz Wagner, Wien, 594 pp.
  • Folmer O, Black M, Hoeh W, Lutz R, Vrijenhoek R (1994) DNA primers for amplification of mitochondrial cytochrome c oxidase subunit I from diverse metazoan invertebrates. Molecular Marine Biology and Biotechnology 3: 294‑299.
  • Hebert PDN, Cywinska A, Ball S, deWaard JR (2003) Biological identifications through DNA barcodes. Proceedings of the Royal Society of London. Series B: Biological Sciences 270 (1512): 313‑321. https://doi.org/10.1098/rspb.2002.2218
  • He JH, Chen XX, Ma Y (2000) Hymenoptera Braconidae. Fauna Sinica. Insecta Vol. 18. Science Press, Beijing.
  • He JY, van Achterberg C, He JH, Tang P, Chen XX (2025) The genus Phanerotomella Szépligeti (Insecta, Hymenoptera, Braconidae, Cheloninae) from China, with description of sixteen new species. European Journal of Taxonomy 1002: 1‑130. https://doi.org/10.5852/ejt.2025.1002.2949
  • Herrich-Schäffer G (1838) Faunae Insectorum Germaniae initiae oder Deutschlands Insecten. Hymenoptera. Auseinandersetzung der Gattung Chelonus (Jurine). Heft 154. Gottlieb August Wilhelm Herrich-Schäffer, Regensburg, 24 pp.
  • Katoh K, Standley DM (2013) MAFFT Multiple Sequence Alignment Software Version 7: Improvements in Performance and Usability. Molecular Biology and Evolution 30 (4): 772‑780. https://doi.org/10.1093/molbev/mst010
  • Kumar S, Stecher G, Li M, Knyaz C, Tamura K (2018) MEGA X: Molecular Evolutionary Genetics Analysis across Computing Platforms. Molecular Biology and Evolution 35 (6): 1547‑1549. https://doi.org/10.1093/molbev/msy096
  • Long KD, Belokobylskij SA (2004) A preliminary list of the Braconidae (Hymenoptera) of Vietnam. Russian Entomological Journal 12 (4): 385‑398.
  • Minh BQ, Schmidt HA, Chernomor O, Schrempf D, Woodhams MD, Von Haeseler A, Lanfear R (2020) IQ-TREE 2: New models and efficient methods for phylogenetic inference in the genomic era. Molecular Biology and Evolution 37 (5): 1530‑1534. https://doi.org/10.1093/molbev/msaa015
  • Nakamura T, Yamada KD, Tomii K, Katoh K (2018) Parallelization of MAFFT for large-scale multiple sequence alignments. Bioinformatics 34 (14): 2490‑2492. https://doi.org/10.1093/bioinformatics/bty121
  • Puillandre N, Lambert A, Brouillet S, Achaz G (2012) ABGD, Automatic Barcode Gap discovery for primary species delimitation. Molecular Ecology 21 (8): 1864‑1877. https://doi.org/10.1111/j.1365-294X.2011.05239.x
  • Sigwalt B (1878) Le genre Phanerotomella Szépligeti généralités. Nouvelles espèces du sud-est asiatique (Hym. Braconidae). Annales de la Société Entomologique de France. (N.S.) 14 (4): 715‑725. https://doi.org/10.1080/21686351.1978.12278714
  • van Achterberg C (1988) Revision of the subfamily Blacinae Foerster (Hymenoptera: Braconidae). Zoologische Verhandelingen Leiden 249: 1‑324.
  • van Achterberg C (1990) Revision of the western Palaearctic Phanerotomini (Hymenoptera: Braconidae). Zoologische Verhandelingen Leiden 255: 1‑106.
  • van Achterberg C (1993) Illustrated key to the subfamilies of the Braconidae (Hymenoptera: Ichneumonoidea). Zoologische Verhandelingen Leiden 283: 1‑189.
  • Xie JM, Chen YR, Cai GJ, Cai RL, Hu Z, Wang H (2023) Tree visualization by one table (tvBOT): A web application for visualizing, modifying and annotating phylogenetic trees. Nucleic Acids Research 51 (W1). https://doi.org/10.1093/nar/gkad359
  • Zettel H (1989) Die Gattung Phanerotomella Szépligeti (Hymenoptera: Braconidae, Cheloninae). Linzer biologische Beiträge 21 (1): 15‑142.
  • Zhang J, Kapli P, Pavlidis P, Stamatakis A (2013) A general species delimitation method with applications to phylogenetic placements. Bioinformatics 29 (22): 2869‑2876. https://doi.org/10.1093/bioinformatics/btt499