BRYOMOLECULES - D1.2 List of new liverworts collected

In this deliverable, we present liverworts which have been selected and collected for downstream analyses in the BRYOMOLECULES project. Plant material has been mostly collected from Sweden, as well as from Iceland and Italy. The list includes collection information, such as sampling place, coordinates, date of sampling, and collector names.
Sterile accessions have been achieved by surface sterilisation of either sporophytes or vegetative plant material with a 0.05% solution of Sodium Dichloroisocyanurate for 1-2 minutes, followed by washing with sterile deionised water. Accessions are maintained on Petri dishes with 1% agar in a medium as described by Rudolph et al. (1988). The medium was 2 times the original formulation with an addition of 12 mg of the fungicide Benomyl per litre to reduce the risk of fungal contamination. If needed, samples have been treated to eliminate infections by cyanobacteria using the antibiotic erythromycin. Collections are maintained in a culture room under artificial LED-tube light, 16/8 day/night cycles and a mean temperature of 20° C.
The collection contains a wide range of liverworts, including both thallous species and leafy liverworts. Taxon names are provided according to Hodgetts et al. (2020). Eventual synonyms used at the moment of collection are listed. The third column indicates what are the accessions for which stable sterile cultures have been obtained. The fourth column indicates whether multiple accession for the same taxon where collected. The fifth column indicates whether the corresponding taxon was used for transcriptomics analyses (See D3.1).

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