Developmental transcriptional atlas of the Drosophila melanogaster VNC (all - loom version)

This object contains scRNA-seq counts data of the Drosophila malanogaster VNC atlas presented in "A Developmental Atlas of the Drosophila Nerve Cord Uncovers a Global Temporal Code for Neuronal Identity" (Cachero et al. 2025, doi.org/10.1101/2025.07.16.664682). This dataset comprises all cells, inclusing neurons and glia. Data were collected from 92 individual animals, covering four developmental stages (6, 24, 36 and 48 hours after puparium formation) and both sexes. Each stage–sex condition is represented by 5 to 18 biological replicates, covering between 5 to 11 different genotypes.

Two umap embeddings are stored in the object:

• UMAPallages: calculated before stage integration
• umap: calculated after integration

Cellular metadata:

• Sex: sex of the animal the cell originated from
• Stage: developmental stage of the animal the cell originated from
• CellType: can be neuron, glia or other