There is a newer version of the record available.

Published August 31, 2025 | Version 0.1
Dataset Open

Bulk RNAseq of microglia in vitro cultures

Creators

  • 1. Laboratory of Molecular Neurogenetics, Department of Experimental Medical Science, Wallenberg Neuroscience Center and Lund Stem Cell Center, BMC A11, Lund University, 221 84 Lund, Sweden.
  • 2. Department of Clinical Neurosciences, University of Cambridge and Department of Pathology, Cambridge University Hospitals NHS Foundation Trust, Cambridge, UK
  • 3. Institute for Systems Genetics, Department of Neuroscience and Physiology, NYU Langone Health, New York, NY 10016, USA. Neuroscience Institute, NYU Grossman School of Medicine, New York, NY 10016, USA.
  • 4. Novo Nordisk Foundation Center for Stem Cell Medicine (reNEW) and Department of Neuroscience, University of Copenhagen, Copenhagen, Denmark.

Description

This Zenodo deposit contains a publicly available description of the Dataset:

Title: "Bulk RNAseq of microglia in vitro cultures".

Description: This dataset includes bulk RNAseq (2 x 150bps reads; Illumina TruSeq Stranded mRNA library prep kit with poly-A selection) of differentiated human pluripotent stem cells (hPSCs) into microglia. Briefly, hES (H9) and hiPSCs (KOLF2.1) underwent mesodermal induction via embryoid body formation, followed by hemogenic endothelium induction and myeloid differentiation to generate primitive monocyte-like precursors. These precursors were then matured into microglia by supplementing with key neuron-derived factors that promote microglial identity, thereby recapitulating human microglia in monoculture. A detailed protocol can be found at doi: dx.doi.org/10.17504/protocols.io.14egnr8ezl5d/v1.

This dataset is made available to researchers via the ASAP CRN Cloud: cloud.parkinsonsroadmap.org. Instructions for how to request access can be found in the User Manual.

This research was funded by the Aligning Science Across Parkinson's Collaborative Research Network (ASAP CRN), through the Michael J. Fox Foundation for Parkinson's Research (MJFF).

This Zenodo deposit was created by the ASAP CRN Cloud staff on behalf of the dataset authors. It provides a citable reference for a CRN Cloud Dataset

Files

jakobsson-invitro-bulk-rnaseq-microglia_README.pdf

Files (7.1 kB)

Additional details

Funding

Aligning Science Across Parkinson's
Activation of transposable elements as a trigger of neuroinflammation in Parkinson’s disease ASAP-000520

References