NFDI4BIOIMAGE
Published September 15, 2025 | Version v1
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NFDI4BIOIMAGE Calendar November 2025

Creators

  • 1. Institute of Neurology (Edinger Institute), University Hospital, Goethe University, Frankfurt, Germany

Contributors

Data curator:

  • 1. ROR icon Heinrich Heine University Düsseldorf
  • 2. ROR icon NFDI4BIOIMAGE

Description

Image from the NFDI4BIOIMAGE Calendar November 2025.

The image shows a perivascular accumulation of B cells, T cells and plasma cells in a human brain tumor. These structures, also known as tertiary lymphoid structures, are sites of lymphocyte clonal expansion and plasma cell formation. The study aims to determine the clinical relevance and immunological function of tertiary lymphoid structures in human primary brain tumors.

Image Metadata (using REMBI template):

Study

Study type

Immunomonitoring study in human oncology

Study Component

Imaging method

COMET™ highplex seq-IF staining and scanning system, HORIZON™ Viewer (Lunaphore Technologies, SA)

Biosample

Biological entity

Tertiary lymphoid structure in glioblastoma

Organism

Homo sapiens

Specimen

Location within biosample

Tumor (glioblastoma)

Preparation method

FFPE sample, automatic sequential-IF using COMET™ (Lunaphore Technologies, SA)

Signal/contrast mechanism

HORIZON™ Viewer (Lunaphore Technologies, SA)

Channel 1 - content

Alexa Fluor Plus 555, red

Channel 1 - biological entity

CD20 - B-cells

Channel 2 - content

Alexa Fluor Plus 647, green

Channel 2 - biological entity

CD3 - T-cells

Channel 3 - content

Alexa Fluor Plus 555, white

Channel 3 - biological entity

CD163 - anti-inflammatory macrophages (M2-like)

Channel 4 - content

Alexa Fluor Plus 647, magenta

Channel 4 - biological entity

MZB-1 - Marginal zone B and B1 cell-specific protein, MEDA-7 - plasma cells, memory B-cells

Channel 5 - content

Alexa Fluor Plus 647, orange

Channel 5 - biological entity

NF- Neurofilament - intermediate filaments around the axons

Channel 6- content

Alexa Fluor Plus 555, cyan

Channel 6 - biological entity

GAP43 - Neuromodulin, neuronal growth-associated protein 43 - neurons

Channel 7 - content

Alexa Fluor Plus 555, blue

Channel 7 - biological entity

vWF - von-Willebrand-Factor - endothelial cells

Image acquisition

Instrument attributes

COMET™ highplex seq-IF staining and scanning system v.1.1.1.0 (Lunaphore Technologies, SA)

Image acquisition parameters

COMET™ acquisition software                                                                                                                                                                                           

Image data

Pixel size

0.23 µm/pixel

Image size

Width 11986 pixels - 2.76 mm
Height 11514 pixels - 2.65 mm

Pixel bit depth

16-bit                                                                                                                                                                                                                                                                             

Channel information

Displayed are 7 markers out of the highplex IF-panel; number of channels 43 (including autofluorescence)

Submitted via NFDI4Immuno

Files

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Additional details

Related works

Is published in
Preprint: 10.1101/2024.07.04.602038 (DOI)