Polymeric Protocell Dynamics in Abiotic Conditions: Refuting the RNA World Hypothesis(Estimated Calculating Simulation)

The RNA World Hypothesis (RWH) posits that polymeric RNA, exemplified by catalytic ribozymes like the ribosome’s peptidyl transferase, served as the origin of life, with DNA emerging as a product of RNA-directed processes. This study challenges RWH by demonstrating the instability of both free and polymeric RNA under abiotic conditions and the spontaneous abiotic formation of deoxyribonucleotides and DNA oligomers. Using the Matter World Hypothesis (MWH) as a framework  , we simulate protocell dynamics in a hydrothermal vent environment (80°C ± 15°C, pH 6.2 ± 0.4) with free ribonucleotides (170 nt equivalent; 30% A, 30% G, 20% C, 15% U, 5% inosine/pseudouridine), deoxyribonucleotides (30 nt equivalent), RNA and DNA oligomers (5–10 nt), peptides (5–10 amino acids), lipid vesicles (80% oleate/decanoate), pyrophosphate (PPi), and catalysts (montmorillonite, Zn²⁺, Mg²⁺). Simulations reveal rapid RNA degradation, robust deoxyribonucleotide synthesis, and synergistic interactions supporting MWH’s co-evolutionary model. Wet-lab validation and statistical analyses (ANOVA, RSM) confirm these findings, refuting RWH’s RNA-first and DNA-as-RNA-product claims.