Published August 15, 2025
| Version v1
Dataset
Open
Structural basis for binding of RILPL1 to TMEM55B reveals a lysosomal platform for adaptor assembly through a conserved TBM motif
Authors/Creators
-
1.
MRC Protein Phosphorylation and Ubiquitylation Unit
Description
Excel raw files and GraphPad Prism files related to graphs for Figure 1F, Figure 3B and 3D, Figure 3E, Figure 4B-F, Supplementary Figure S3B, Supplementary Figure S5A are presented here.
Table of contents:
| Figure Number | Raw File Name | GraphPad Prism File Name | Description |
| 1F | 1F-Graph-Raw-File | 1F | Bar plots describes the binding of RILPL1 with TMEM55B affected due to the presence of G396L and E398K point mutations. |
| 3B and 3D | 3B-3D_Violin-Plots-Raw-File | 3B-3D_Violin-Plots | Violin plots generated from the mass spectrometry experiment 1 [TMEM55B WT/ LRRK2 Y1699C/ Rab8A Q67L vs TMEM55B R151E/ LRRK2 Y1699C/ Rab8A Q67L] and experiment 2 [TMEM55B WT/ LRRK2 Y1699C/ Rab8A Q67L vs TMEM55B WT/ LRRK2 Y1699C D2017A/ Rab8A Q67L] |
| 3E | 3E_Graph-Raw-File | 3E | Representative graph shows that the interaction of RILPL1 with TMEM55B depends of kinase activity of LRRK2 and the presence of phosphoryted Rabs. |
| 4B to 4F | 4B-F_Graph-Raw-File | 4B-F | Bar plots describes the interaction of TMEM55B with JIP3, JIP4, OCRL, WDR81, and TBC1D9B depends on the TBM. Mutation in this motif at the critical residue disrupts the binding. |
| Supplementary Figure S3B | Sup3B_Raw-File | Sup3B | Bar plot shows that there is an additional interface presents in JIP4 where a mutation K103E of TMEM55B affects the binding of TMEM55B with JIP4 but not RILPL1. |
| Supplementary Figure S5A | Sup5A_Raw-File | Sup5A | Apart from the R151 in TMEM55B, E116 residue is critical for interaction with RILPL1. |
Files
Files
(308.1 kB)
| Name | Size | Download all |
|---|---|---|
|
md5:800a4aaedb4b994eff2cb8f9c92983fd
|
11.1 kB | Download |
|
md5:c5725a385341580b805ed0e500506ba5
|
10.2 kB | Download |
|
md5:2bd490382d2785bb00489ac85a6d01a3
|
14.7 kB | Download |
|
md5:e4bcd4598d3d0b6cae7495f165d080a4
|
112.0 kB | Download |
|
md5:b52f560b6dfb6ccc6ef48d9d66baf839
|
28.4 kB | Download |
|
md5:c7d52ced28406b5a5bcee8c9573ee11e
|
11.5 kB | Download |
|
md5:9127aaa236be00ef94dab1269f991ca9
|
41.8 kB | Download |
|
md5:01a6c8ac7a75eea1ccd5364083ba21e5
|
14.4 kB | Download |
|
md5:4133077a9be0875065fdce8d5cefb6ef
|
24.5 kB | Download |
|
md5:ebc908589d077cc687c1c25d097ef9c3
|
11.7 kB | Download |
|
md5:f82715bd1d783e9d4b807b32ce766737
|
15.7 kB | Download |
|
md5:739ba61904fcdc675dabd5e8ce1ba87b
|
12.2 kB | Download |