Detection of abortifacient agents in domestic ruminants, with a specific focus on Coxiella burnetii
Authors/Creators
- 1. Small Ruminant Health Department, Royal GD, Deventer, 7400 AA, The Netherlands
- 2. Penrith Veterinary Investigation Centre, Animal and Plant Health Agency (APHA), Cumbria, CA11 9RR, UK
- 3. University of Clermont Auvergne, INRAE, VetAgro Sup, UMR EPIA,, Saint-Genès Champanelle, France
- 4. Sciensano, Belgian Institute for Health, Brussels, Belgium
- 5. ANSES, Sophia Antipolis Laboratory, Animal Q fever Unit, Sophia Antipolis, France
- 6. Friedrich-Loeffler-Institut, Institute of Bacterial Infections and Zoonoses, Jena, 07743, Germany
- 7. Moredun Research Institute, Penicuik, EH26 0PZ, UK
- 8. Animal Health Department, NEIKER – Basque Institute for Agricultural Research and Development, Bizkaia, 48160 Derio, Spain
Description
Q fever is a widespread zoonotic disease caused by the bacterium, Coxiella burnetii. In ruminants, C. burnetii can cause abortions, stillbirths, premature births, and weak offspring. As part of the EU-funded Q-Net-Assess International Coordination of Research on Infectious Animal Diseases (ICRAD)-project, aimed at generating the most comprehensive understanding of C. burnetii genetic variation to date and determining the implications of this genetic variation for zoonotic risk, pathogenicity and control of C. burnetii infection, we have reviewed the protocols reported by the different project partners and/or countries to diagnose abortion in domestic ruminants. As a result of this review, we have developed guidelines for the detection of abortifacient agents in domestic ruminants, with a special focus on C. burnetii. They include a description of the essential and complementary samples needed for a definitive diagnosis, the analytical techniques to be used, and the interpretation and validity of each type of sample and technique. The most comprehensive diagnostic approach to identify an infectious agent as the cause of abortion in ruminants would include histopathology, including immunohistochemistry (IHC), on the fetus and placental membranes, complemented by bacteriology, serology, and real-time PCR analyses of different types of samples. For the specific diagnosis of C. burnetii as the causative agent of abortion, we provide guidelines based on expert opinions for the interpretation of laboratory test results in relation to their diagnostic value.
Q fever, caused by the bacterium Coxiella burnetii, can be transmitted from animals to humans. It may cause reproductive issues in animals, such as cows, sheep, and goats, including miscarriages and weak offspring. The EU-funded Q-Net-Assess project aims to understand the genetic variations in C. burnetii and their impact on disease spread, severity, and control. Researchers in this project reviewed European methods for diagnosing miscarriages in these animals and created guidelines to identify causative agents, focusing on C. burnetii. The guidelines specify the necessary samples, diagnostic techniques, and interpretation of the results. The best diagnostic approach involves examining fetal and placental tissues using immunohistochemistry (IHC) supported by bacteriology, serology, and real-time PCR tests. For diagnosing C. burnetii, expert advice is provided to interpret the lab results.
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References
- Agerholm JS (2013). associated reproductive disorders in domestic animals—a critical review. Acta Vet Scand. doi:10.1186/1751-0147-55-13
- Álvarez-Alonso R, Zendoia II, Barandika JF (2020). Monitoring infection in naturally infected dairy sheep flocks throughout four lambing seasons and investigation of viable bacteria. Front Vet Sci. doi:10.3389/fvets.2020.00352
- Angelakis E, Raoult D (2010). Q fever. Vet Microbiol. doi:10.1016/j.vetmic.2009.07.016
- Arricau-Bouvery N, Souriau A, Bodier C (2005). Effect of vaccination with phase I and phase II vaccines in pregnant goats. Vaccine. doi:10.1016/j.vaccine.2005.04.010
- Babudieri B (1959). Q fever: a zoonosis (1959). Adv Vet Sci.
- Boarbi S, Mori M, Rousset E (2014). Prevalence and molecular typing of in Bulk Tank Milk in Belgian dairy goats, 2009–2013. Vet Microbiol. doi:10.1016/j.vetmic.2014.01.025
- Borel N, Frey CF, Gottstein B (2014). Laboratory diagnosis of ruminant abortion in Europe. Vet J. doi:10.1016/j.tvjl.2014.03.015
- Carrié P, Barry S, Rousset E (2019). Swab cloths as a tool for revealing environmental contamination by Q fever in ruminant farms. Transbound Emerg Dis. doi:10.1111/tbed.13137
- Celina SS, Cerný J (2022). in ticks, livestock, pets and wildlife: a mini-review. Front Vet Sci. doi:10.3389/fvets.2022.1068129
- Clark NJ, Soares Magalhães RJ (2018). Airborne geographical dispersal of Q fever from livestock holdings to human communities: a systematic review and critical appraisal of evidence. BMC Infect Dis. doi:10.1186/s12879-018-3135-4
- Clune T, Beetson S, Besier S (2021). Ovine abortion and stillbirth investigations in Australia. Aust Vet J. doi:10.1111/avj.13040
- de Bruin A, Janse I, Koning M (2013). Detection of DNA in the environment during and after a large Q fever epidemic in the Netherlands. J Appl Microbiol. doi:10.1111/jam.12163
- de Cremoux R, Rousset E, Touratier A (2012). vaginal shedding and antibody responses in dairy goat herds in a context of clinical Q fever outbreaks. FEMS Immunol Med Microbiol. doi:10.1111/j.1574-695X.2011.00893.x
- Duron O, Sidi-Boumedine K, Rousset E (2015). The importance of ticks in Q fever transmission: what has (and has not) been demonstrated?. Trends Parasitol. doi:10.1016/j.pt.2015.06.014
- (2010). Development of harmonised schemes for the monitoring and reporting of Q-fever in animals in the European Union. doi:10.2903/sp.efsa.2010.EN-48
- Eldin C, Melenotte C, Mediannikov O (2017). From Q fever to infection: a paradigm shift. Clin Microbiol Rev. doi:10.1128/CMR.00045-16
- Fournier PE, Marrie TJ, Raoult D (1998). Diagnosis of Q fever. J Clin Microbiol. doi:10.1128/JCM.36.7.1823-1834.1998
- Gisbert P, Garcia-Ispierto I, Quintela LA (2024). and reproductive disorders in cattle: a systematic review. Animals (Basel). doi:10.3390/ani14091313
- Givens MD, Marley MSD (2008). Infectious causes of embryonic and fetal mortality. Theriogenology. doi:10.1016/j.theriogenology.2008.04.018
- Hecker YP, González-Ortega S, Cano S (2023). Bovine infectious abortion: a systematic review and meta-analysis. Front Vet Sci. doi:10.3389/fvets.2023.1249410
- Hoffmann B, Scheuch M, Höper D (2012). Novel orthobunyavirus in Cattle, Europe, 2011. Emerg Infect Dis. doi:10.3201/eid1803.111905
- Hogerwerf L, Koop G, Klinkenberg D (2014). Test and cull of high risk infected pregnant dairy goats is not feasible due to poor test performance. Vet J. doi:10.1016/j.tvjl.2014.02.015
- Hurtado A, Alonso E, Aspiritxaga I (2017). Environmental sampling coupled with real-time PCR and genotyping to investigate the source of a Q fever outbreak in a work setting. Epidemiol Infect. doi:10.1017/S0950268817000796
- Hurtado A, Zendoia II, Alonso E (2023). A Q fever outbreak among visitors to a natural cave, Bizkaia, Spain, December 2020 to October 2021. Euro Surveill. doi:10.2807/1560-7917.ES.2023.28.28.2200824
- Jansen W, Cargnel M, Boarbi S (2022). Belgian Bulk Tank Milk surveillance program reveals the impact of a continuous vaccination protocol for small ruminants against . Transbound Emerg Dis. doi:10.1111/tbed.14273
- Jones RM, Hertwig S, Pitman J (2011). Interlaboratory comparison of real-time polymerase chain reaction methods to detect , the causative agent of Q fever. J Vet Diagn Invest. doi:10.1177/104063871102300118
- Jourdain E, Duron O, Barry S (2015). Molecular methods routinely used to detect in ticks cross-react with -like bacteria. Infect Ecol Epidemiol. doi:10.3402/iee.v5.29230
- Klee SR, Tyczka J, Ellerbrok H (2006). Highly sensitive real-time PCR for specific detection and quantification of . BMC Microbiol. doi:10.1186/1471-2180-6-2
- Körner S, Makert GR, Ulbert S (2021). The prevalence of in hard ticks in Europe and their role in Q fever transmission revisited-a systematic review. Front Vet Sci. doi:10.3389/fvets.2021.655715
- Lurier T, Rousset E, Gasqui P (2021). Evaluation using latent class models of the diagnostic performances of three ELISA tests commercialized for the serological diagnosis of infection in domestic ruminants. Vet Res. doi:10.1186/s13567-021-00926-w
- Marrie TJ, Durant H, Williams JC (1988). Exposure to parturient cats: a risk factor for acquisition of Q fever in Maritime Canada. J Infect Dis. doi:10.1093/infdis/158.1.101
- Maurin M, Raoult D (1999). Q fever. Clin Microbiol Rev. doi:10.1128/CMR.12.4.518
- Mee JF (2023). Invited review: bovine abortion-Incidence, risk factors and causes. Reprod Domest Anim. doi:10.1111/rda.14366
- Mori M, Boarbi S, Michel P (2013). and infectious potential of : a study on Belgian livestock isolates. PLoS One. doi:10.1371/journal.pone.0067622
- Periyasamy D, Huang Y, Hill JE (2025). Targeted syndromic next-generation sequencing panel for simultaneous detection of pathogens associated with bovine reproductive failure. J Clin Microbiol. doi:10.1128/jcm.01433-24
- Plummer PJ, McClure JT, Menzies P (2018). Management of infection in livestock populations and the associated zoonotic risk: a consensus statement. J Vet Intern Med. doi:10.1111/jvim.15229
- Roest HJ, van Gelderen B, Dinkla A (2012). Q fever in pregnant goats: pathogenesis and excretion of . PLoS One. doi:10.1371/journal.pone.0048949
- Rousset E (2021). PCR detection and/or quantification of for the diagnosis of abortion in ruminants.
- Rousset E, Berri M, Durand B (2009). shedding routes and antibody response after outbreaks of Q fever-induced abortion in dairy goat herds. Appl Environ Microbiol. doi:10.1128/AEM.00690-08
- Rousset E, Sidi-Boumedine K, Niemczuk K (2018). Q Fever.
- Thomas KM, Kibona T, Claxton JR (2022). Prospective cohort study reveals unexpected aetiologies of livestock abortion in northern Tanzania. Sci Rep. doi:10.1038/s41598-022-15517-8
- Van den Brom R, Lievaart-Peterson K, Luttikholt SJM (2012). Abortion in small ruminants in the Netherlands between 2006–2011. Tijdschr Diergeneeskd.
- Van den Brom R, Santman-Berends I, Dijkman R (2021). An accessible diagnostic toolbox to detect bacterial causes of ovine and caprine abortion. Pathogens. doi:10.3390/pathogens10091147
- Van den Brom R, Santman-Berends I, Luttikholt SJM (2015). Bulk tank milk surveillance as a measure to detect shedding dairy goat farms in the Netherlands between 2009 and 2014. J Dairy Sci. doi:10.3168/jds.2014-9029
- Van den Wijngaard CC, Dijkstra F, van Pelt W (2011). In search of hidden Q-fever outbreaks: linking syndromic hospital clusters to infected goat farms. Epidemiol Infect. doi:10.1017/S0950268810001032
- (2024). Terrestrial code online access.
- Yessinou RE, Katja MS, Heinrich N (2022). Prevalence of -infections in ticks - review and meta-analysis. Ticks Tick Borne Dis. doi:10.1016/j.ttbdis.2022.101926