There is a newer version of the record available.

Published March 13, 2024 | Version v1.1.0
Software Open

epi2me-labs/wf-single-cell: v1.1.0

Authors/Creators

  • 1. Oxford Nanopore Technologies
  • 2. @nanoporetech
  • 3. @Birkbeck
  • 4. @nanoporetech, @epi2me-labs

Description

Added

  • full_length_only parameter to process only full length reads (default: true).
  • Trim adapters, barcodes and UMIs from reads before alignment.
  • Memory directive for umap process to prevent parallel processes from using too much memory.

Changed

  • Orient 3prime/multiome reads to mRNA sense to avoid need to flip later.
  • Default umap_n_repeats lowered to 3.
  • Genome reference alignment done by chunk.

Fixed

  • Issue where splice junctions were searched for on incorrect strand.

Files

epi2me-labs/wf-single-cell-v1.1.0.zip

Files (22.5 MB)

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md5:3c96349fe3b5e45d6f7efe1aaf376b6b
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Additional details

Related works

Is supplement to
Software: https://github.com/epi2me-labs/wf-single-cell/tree/v1.1.0 (URL)

Software

Repository URL
https://github.com/epi2me-labs/wf-single-cell