ACEMBLing multigene expression vectors by recombineering

Multiprotein complexes are an emerging focus of contemporary biological research efforts (1). Molecular and structural studies of multiprotein assemblies are often handicapped by the low abundance and heterogeneous nature of most of these complexes in their native hosts, thus inhibiting direct extraction. Recombinant methods that can achieve overproduction of these multiprotein complexes are therefore often a crucial prerequisite for their study.