Published March 30, 2015 | Version v1
Journal article Open

Preparation of cell-sized water-in-oil droplets for in vitro reconstitution of biological processes in cellular compartments

Authors/Creators

  • 1. ScientificProtocols.org

Description

This protocol presents a method for encapsulation of purified proteins into cell-sized water-in-oil droplets surrounded by a phospholipid monolayer, which can be broadly applied to studies to reconstitute biological processes in cellular compartments.

Files

protocol.md

Files (11.6 kB)

Name Size Download all
md5:d32adfe9c005d37d1adf3b8e360d3c27
11.6 kB Preview Download