Isolation of extracellular vesicle RNA from bile

Extracellular vesicles can be isolated from different types of body fluids such as bile. These extracellular vesicles may contain protein, mRNA, and non-coding RNA. Extracellular vesicle RNA isolated from bile could be useful as a biomarker of biliary tract disease. This protocol presents sample collection, process, and extracellular RNA isolation of bile using three different commercially available kits.