IDENTIFIKASI ANTOSIANIN EKSTRAK STROBERI MENGGUNAKAN NATURAL DEEP EUTECTIC SOLVENTS (NADES) MELALUI ANALISIS KEMOMETRIK DAN PENGUJIAN TOKSISITAS SECARA IN VIVO

Strawberry (Fragaria x ananassa) is a natural bioactive source rich in phenolic compounds, especially

anthocyanins. This study aims to compare the chemical characteristics and toxicity of strawberry extracts

obtained using NADES and conventional solvents. Functional group analysis was performed using FTIR,

while differences in chemical patterns were evaluated through PCA chemometric analysis. Qualitative and

quantitative profiles of anthocyanin compounds were analyzed using LC-MS/MS, and toxicity testing was

evaluated based on LD50 values using the OECD 423 method. FTIR results showed significant differences

in functional group absorption between strawberry extracts using NADES and conventional solvents,

especially in the O-H, C=O, and aromatic C=C groups. PCA of the FTIR spectrum can group strawberry

extracts based on solvent type. LC-MS/MS analysis showed that the anthocyanin content in strawberry

extracts using NADES was higher than in conventional solvents. Toxicity testing showed that the LD50 value

of strawberry extract using NADES LD50 < 5000 mg/kg, while conventional solvents were LD50 < 2000

mg/kg. The results showed that NADES has the potential to be a safe alternative solvent for the extraction

of bioactive compounds from strawberrie