Bionano Irys Optical Genome Mapping data of Embleya australiensis MST-111070 isolate MST-111070v14

The "Strep_1_2015-12-11_13_14.tar.gz" file contains the full original package (made available to us by the technology provider and prior any further analysis) with the raw data of the optical mapping performed with Bionano Irys (Bionano Genomics, San Diego, USA) technology on genomic DNA of the actinomycete strain Embleya australiensis MST-111070 isolate MST-111070v14, that led to the publication of a high-quality genome assembly of this organism (https://doi.org/10.1101/2025.07.03.662523  and NCBI BioProject PRJNA1219506). As described in the methods section of the referred work: "Bacterial cells were prepared from a liquid culture following supplier’s instructions; DNA-extraction, labelling and data collection including processing of images to extract BNX files with molecules information, was commissioned to the Earlham Institute (Norwich Research Park, Norwich, NR4 7UZ, United Kingdom). We received BNX files with Bionano molecules information for a high-resolution map of the distribution of the recognition site for nickase Nt.BspQI (GCTCTTC)." The BNX files as well as the original images are provided in this package.

The file "NCBI_SUB14353019.zip" contains the final DNA sequence assembly submitted to NCBI, and includes the full assembly in FASTA format which can be used as refference for the Bionano data analysis. It is also included the genome assembly annotated by NCBI in Genbank format, and the .acc file with the allocated accesion numbers.

All these original raw data and DNA sequence assembly allow the reproduction of our analysis and test of our hypothesis and conclussions, with current software or other available in future.