Injection of virus vectors into the neocortical layer 1

Injection of the reagents, such as virus vectors, anterograde and retrograde tracers, antagonists and agonists, into the brain is an important method in neuroscience. Since the injection site of solutions is generally restricted to a target region, such as thalamic nuclei and cortical areas, the functions, connections and morphology of the target regions can be examined by the injections of reagents.