Published July 14, 2025 | Version v1
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THE PATCH CLAMP METHOD CONFIGURATIONS AND PROTOCOLS FOR ASSESSING NEURONAL EXCITABILITY

Authors/Creators

  • 1. Medical University – Pleven, Bulgaria

Contributors

  • 1. ROR icon University of Forestry

Description

Electrophysiology serves as an indispensable instrument in the investigation of the physiological and pathological characteristics of electrically active cells and their interconnected networks. The patch clamp method exhibits remarkable flexibility and can be employed in numerous configurations to examine a spectrum of properties.
However, adequate protocols are essential for precise measurements, ensuring the accuracy and repro-ducibility of experiments. The diversity of cell types requires tailored approaches, adding complexity and highlighting the need for robust protocols to enrich our understanding of neural functions.
The protocols proposed by the author represent a thoughtful contribution to the study of CA1 hippocam-pal neurons. They are designed to align with the physiological characteristics of these neurons, allowing suf-ficient time for cellular recovery following each electrical stimulus, avoiding artifacts caused by cellular fa-tigue. The protocols take into consideration the properties of the voltage-gated channels expressed, proving to be suitable for the study of neuronal excitability.

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Dates

Issued
2025-07-07

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