General information

The data belong to the article "Acute changes in macronutrient stoichiometry alter nitrate uptake in benthic biofilms".

The data are from a lab experiment conducted in Gainesville, Florida, USA. We took sediment samples from four streams of different land-use and background macronutrient stoichiometry. We manipulated the DOC:DIN:PO4-P ratio in a full-factorial design by adding sodium acetate as DOC source and/or sodium nitrate.

Data files

The files can be grouped into the three categories of raw data, R code and output file. 

Raw data:

• Catchment.csv contains information about the catchment of the four sampling sites. This file is loaded into the Dat_Analysis.Rmd file for further analysis.
• Data.csv contains information about the benthic biofilm parameters and measured initial and final water macronutrient concentrations. This file is loaded into the Data_Analysis.Rmd file for further analysis.
• DataTernary.csv contains the coordinates of the depletion zones, which are used in Data_Analysis.Rmd
• PhysChem.csv contains data on physical and chemical parameters measured during the sediment sampling in the streams. This file is loaded into the Data_Analysis.Rmd file for further analysis.
• Sediment_Dry_weight.csv contains the weights of sediment during the incubation. This file is loaded into the Data_Analysis.Rmd file for further analysis.

R code:

• Data_Analysis.Rmd loads the raw data files and does the data analysis for th emanuscript. Further information can be found in the file.

Output file:

• Data_Analysis.html is the output file from the R Markdown file. 

R setup

The R code Dana_Analysis.Rmd was developed with R version 4.4. and uses the following R libraries:

• dplyr
• tidyr
• RColorBrewer
• ggcorrplot
• corrplot
• vegan
• performance
• parameters
• ggtern
• knitr
• multcompView
• broom
• emmeans
• ggplot2
• ggpubr
• ggeffects
• ggh4x
• sp
• mapview