Involvement of Microglia in RGC Injury Induced by IOP Elevation in a Rat Ex Vivo Model
Authors/Creators
- 1. Department of Ophthalmology, Tohoku University Graduate School of Medicine, Japan
- 2. Department of Psychiatry, Washington University School of Medicine, St. Louis, MO, USA
- 3. Aoba Eye Clinic, Akita, Japan
- 4. Department of Advanced Ophthalmic Medicine, Tohoku University Graduate School of Medicine, Sendai, Japan
- 5. Department of Retinal Disease Control, Tohoku University Graduate School of Medicine, Japan
- 6. Department of Retinal Disease Control, Tohoku University Graduate School of Medicine, Sendai, Japan
Description
This dataset supports the findings of the manuscript titled:
"Involvement of microglia in retinal ganglion cell injury induced by IOP elevation in a rat ex vivo acute glaucoma model."
In this study, we used a rat ex vivo model of acute intraocular pressure (IOP) elevation to investigate the role of microglia in retinal ganglion cell (RGC) injury. Our results demonstrate that pressure-induced activation of the TLR4-NLRP3 inflammasome cascade contributes to microglial proliferation and IL-1β production, leading to RGC apoptosis. Pharmacological depletion of microglia using the CSF-1R inhibitor PLX5622 suppressed this inflammatory pathway and preserved RGCs.
The uploaded files include:
- Raw and normalized data from flat-mounted immunostaining (RGC and microglia counts), Western blot membranes (protein expression), TUNEL-stained cryosections (apoptosis), and Epon-embedded sections (NFL thickness and NDS evaluation).
- Source data used to generate the figures.
This dataset is made available to promote transparency and reproducibility in glaucoma research, particularly in studies involving retinal neuroinflammation and microglial modulation.
Files
Minimal dataset.zip
Files
(195.8 MB)
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